product summary
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company name :
HUABIO
product type :
antibody
product name :
DYKDDDDK Tag (FLAG)
catalog :
M1403-2
quantity :
100μl
price :
360.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
A2-A4
application :
western blot, ELISA, immunoprecipitation
more info or order :
image
image 1 :
Western blot analysis of DYKDDDDK Tag on Flag-tagged recombinant protein Flag-Ki67 and Ki67-Flag at different cell lysates level.
Predicted band size: 35 kDa;
Observed band size: 35 kDa;
Exposure time: 60 seconds;
SDS-PAGE gel concentration:12%
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1403-2, 1/20,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/200,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of DYKDDDDK Tag on Flag-tagged recombinant protein 2xflag-Histone H3.1 at different dilutions of HeLa cells with anti-DYDDDDK antibody (M1403-2).
Lane 1: Non-transfected HeLa cell lysates (1/2000)
Lane 2~4: Transfected HeLa cell lysates (1/2500; 1/5000; 1/10,000)
Predicted band size: 15 kDa;
Observed band size: 18 kDa;
Exposure time: 30 seconds;
SDS-PAGE gel concentration:12%.
Cell lysate at 10ug per lane.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1403-2) was used at different dilutions in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/200,000 dilution was used for 1 hour at room temperature.
image 3 :
Immunoprecipitation analysis of N-Flag in HeLa cells.
Lane 1: HeLa transfected with DYKDDDDK-tagged human Histone H3.1 expression vector whole cell lysate (input).
Lane 2: M1403-2 IP in HeLa transfected with DDDDK-tagged human Histone H3.1 expression vector whole cell lysate.
Lane 3: Mouse IgG (HA1027) instead of M1403-2 in HeLa transfected with DYKDDDDK-tagged human Histone H3.1 expression vector whole cell lysate.
Predicted band size: 19 kDa;
Observed band size: 19 kDa;
Exposure time: 60 seconds;
SDS-PAGE gel concerntration: 15%.
DYKDDDDK Tag was immunoprecipitated from 0.5 mg Hela transfected with human 2x Flag- Histone H3.1 expression vector whole cell lysate with M1403-2 2 ug/mL. Western blot was performed from the immunoprecipitate using M1403-2 at 1/5,000 dilution for 1 hour at room temperature. The secondary antibody for IP detection reagent, Goat anti-Mouse IgG-HRP antibody (HA1006), was used at 1:100,000 dilution.
product information
SKU :
M1403-2
Target name :
DYKDDDDK Tag (FLAG)
Species reactivity :
Species independent
Applications :
WB,IP,ELISA,IF-Cell
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide immune sequence is N-DYKDDDDK-C.
Uniprot id :
>
Host :
Mouse
Clone number :
A2-A4
Isotype :
IgG1
Size :
100μl
List Price :
360.00 USD
Storage Buffer :
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein G affinity purified.
Product type :
Mouse monoclonal Antibody
Positive control :
DYKDDDDK Tag recombinant protein, C-terminal FLAG-tagged recombinant protein, N-terminal FLAG-tagged recombinant protein.
Concentration :
2 mg/mL.
Recommended dilutions :
WB: 1:20,000-1:50,000
;IP: 2-5 µg/ml.
;IF-Cell: 1:500-1:10,000
Pic img4 :
https://storage.huabio.cn/huabio/productImg/M1403-2_4.jpg
Pic legend4 :
Immunoprecipitation analysis of C-Flag in HeLa cells.
Lane 1: HeLa transfected with DYKDDDDK-tagged human Siglec15 expression vector whole cell lysate (input).
Lane 2: M1403-2 IP in HeLa transfected with DDDDK-tagged human Siglec15 expression vector whole cell lysate.
Lane 3: Mouse IgG (HA1027) instead of M1403-2 in HeLa transfected with DYKDDDDK-tagged human Siglec15 expression vector whole cell lysate.
DYKDDDDK Tag was immunoprecipitated from 0.5 mg Hela transfected with human Siglec15 expression vector whole cell lysate with M1403-2 2 ug/mL. Western blot was performed from the immunoprecipitate using M1403-2 at 1/2,000 dilution for 1 hour at room temperature. The secondary antibody for IP detection reagent, Goat anti-Mouse IgG-HRP antibody (HA1006), was used at 1:100,000 dilution.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/M1403-2_5.jpg
Pic legend5 :
Immunofluorescent analysis of 2xFlag tagged Histone H3.1 in HeLa cells.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeling DYKDDDDK tag with M1403-2 at 1/500 dilution, followed by iFluor™ 488 Goat anti-mouse IgG antibody (HA1125) at 1/1000 dilution (green).
Pic img6 :
https://storage.huabio.cn/huabio/productImg/M1403-2_6.jpg
Pic legend6 :
Immunocytochemistry analysis of HeLa cells labeling DYKDDDDK Tag (FLAG) with Mouse anti-DYKDDDDK Tag (FLAG) antibody (M1403-2) at 1/10,000 dilution.
HeLa cells, transfected with empty control (top, negative) / Flag-tagged Claudin 18.2 (middle, positive) / Flag-tagged Histone H3.1 (bottom, positive) expression vector, respectively, were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-DYKDDDDK Tag (FLAG) antibody (M1403-2) at 1/10,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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