Lin28B | HUABIO M1312-1 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Lin28B

catalog :

M1312-1

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A2-4-5

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Lin28B on different lysates with Mouse anti-Lin28B antibody (M1312-1) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (negative) Lane 2: JAR cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 27 kDa Observed band size: 35 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1312-1) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

ICC staining Lin28B in NCCIT cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. and counterstained with DAPI in order to highlight the nucleus (blue).

image 3 :

Immunohistochemical analysis of paraffin-embedded human placenta tissue with Mouse anti-Lin28B antibody (M1312-1) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1312-1) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

M1312-1

Target name :

Lin28B

Species reactivity :

Human

Applications :

WB,IF-Cell,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human Lin28B aa 73-250 / 250.

Uniprot id :

Q6ZN17>SwissProt: Q6ZN17 Human

Host :

Mouse

Clone number :

A2-4-5

Isotype :

IgG2a

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

JAR cell lysate, NCCIT, human placenta tissue.

Molecular wt :

Predicted band size: 27 kDa

Subcellular location :

Cytoplasm, nucleus

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:100-1:200 ;IHC-P: 1:1,000

Advanced Validation :

Relative expression (RE)

more info or order :

HUABIO product webpage