product summary
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company name :
HUABIO
product type :
antibody
product name :
Anti-GAPDH Antibody [12D6]
catalog :
M1310-2TR
quantity :
20 uL
price :
99 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
12D6
reactivity :
human, mouse, rat, zebrafish
application :
western blot, immunohistochemistry - paraffin section
more info or order :
product information
SKU :
M1310-2TR
Target name :
Anti-GAPDH Antibody [12D6]
Species reactivity :
Human,Mouse,Rat,Zebrafish,Escherichia coli
Applications :
WB,IF-Cell,IHC-P
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within human GAPDH aa 180-220.
Uniprot id :
SwissProt: P04406 Human;SwissProt: P16858 Mouse;SwissProt: P04797 Rat
Host :
Mouse
Clone number :
12D6
Isotype :
IgG1
Size :
20 uL
List Price :
99 USD
Storage Buffer :
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein G affinity purified.
Product type :
Mouse monoclonal Antibody
Positive control :
Hela cell lysate, A549 cell lysate, HepG2 cell lysate, PC-12 cell lysate, F9 cell lysate, Escherichia coli lysate, D3, A431, zebrafish tissue lysates, human thyroid carcinoma tissue, human colon carcinoma tissue.
Molecular wt :
Predicted band size: 36 kDa
Subcellular location :
Cytoplasm, Nucleus, Membrane.
Concentration :
2 mg/mL.
Recommended dilutions :
WB: 1:2000-1:5,000;IF-Cell: 1:200;IHC-P: 1:600
Pic img4 :
M1310-2_2.jpg
Pic img5 :
Western blot analysis of GAPDH on zebrafish tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M1310-2, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Pic legend5 :
M1310-2_3.jpg
Pic legend6 :
Western blot analysis of GAPDH on different lysates with Mouse anti-GAPDH antibody (M1310-2) at 1/500 dilution.
Lane 1: Escherichia coli lysate
Lane 2: Escherichia coli lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 36 kDa
Observed band size: 36 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1310-2) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature.
Pic img7 :
M1310-2_4.jpg
Pic img8 :
ICC staining of GAPDH in D3 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (M1310-2, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®555 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
Pic legend8 :
M1310-2_5.jpg
Pic legend9 :
ICC staining of GAPDH in A431 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (M1310-2, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®555 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
Pic img10 :
M1310-2_6.jpg
Pic img11 :
Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue with Mouse anti-GAPDH antibody (M1310-2) at 1/600 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1310-2) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic legend11 :
M1310-2_7.jpg
Pic legend12 :
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Mouse anti-GAPDH antibody (M1310-2) at 1/600 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1310-2) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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