TMEM177 | HUABIO M1306-1 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

TMEM177

catalog :

M1306-1

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A6-A11-9

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of TMEM177 on recombinant protein using anti-TMEM177 antibody at 1/1,000 dilution.

image 2 :

Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-TMEM177 antibody (M1306-1) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1306-1) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

ICC staining TMEM177 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

M1306-1

Target name :

TMEM177

Species reactivity :

Human

Applications :

WB,IF-Cell,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human TMEM177 aa 1-311 / 311.

Uniprot id :

Q53S58>SwissProt: Q53S58 Human

Host :

Mouse

Clone number :

A6-A11-9

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

TMEM177 recombinant protein, human liver tissue, HepG2, Hela, MCF-7.

Molecular wt :

Predicted band size: 34 kDa

Subcellular location :

Mitochondrion inner membrane.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:500-1:1,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/M1306-1_4.jpg

Pic legend4 :

ICC staining TMEM177 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/M1306-1_5.jpg

Pic legend5 :

ICC staining TMEM177 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

more info or order :

HUABIO product webpage