beta Tubulin | HUABIO M1305-2 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

beta Tubulin

catalog :

M1305-2

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A1-A4

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of beta Tubulin on Hela cell lysates with Mouse anti-beta Tubulin antibody (M1305-2). Hela cell lysates at 10 µg/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1305-2) at serial dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of beta Tubulin on NIH/3T3 cell lysates with Mouse anti-beta Tubulin antibody (M1305-2). NIH/3T3 cell lysates at 10 µg/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1305-2) at serial dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

image 3 :

Western blot analysis of beta Tubulin on PC-12 cell lysates with Mouse anti-beta Tubulin antibody (M1305-2). PC-12 cell lysates at 10 µg/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1305-2) at serial dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

product information

SKU :

M1305-2

Target name :

beta Tubulin

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human Beta tubulin aa 151-200 / 444.

Uniprot id :

P07437>SwissProt: P07437 Human;SwissProt: P99024 Mouse;SwissProt: P69897 Rat

Host :

Mouse

Clone number :

A1-A4

Isotype :

IgG3

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

Hela cell lysates, NIH/3T3 cell lysates, PC-12 cell lysates, SKOV-3 cells, HeLa, 293, human tonsil tissue, human spleen tissue, human colon tissue, rat brain tissue, NIH/3T3.

Molecular wt :

Predicted band size: 50 kDa

Subcellular location :

Cytoplasm, Cytoskeleton, Microtubule.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:5,000-1:20,000 ;IF-Cell: 1:100-1:500 ;IHC-P: 1:50-1:200 ;FC: 1:100-1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/M1305-2_4.jpg

Pic legend4 :

Western blot analysis of beta Tubulin on different lysates with Mouse anti-beta Tubulin antibody (M1305-2) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: PC-12 cell lysate Lane 5: C6 cell lysate Lane 6: Vero cell lysate Lane 7: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1305-2) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/M1305-2_5.jpg

Pic legend5 :

Immunocytochemistry analysis of HeLa cells labeling beta Tubulin with Mouse anti-beta Tubulin antibody (M1305-2) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-beta Tubulin antibody (M1305-2) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/M1305-2_6.jpg

Pic legend6 :

Immunocytochemistry analysis of 293 cells labeling beta Tubulin with Mouse anti-beta Tubulin antibody (M1305-2) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-beta Tubulin antibody (M1305-2) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/M1305-2_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-beta tubulin antibody. Counter stained with hematoxylin.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/M1305-2_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-beta tubulin antibody. Counter stained with hematoxylin.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/M1305-2_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-beta tubulin antibody. Counter stained with hematoxylin.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/M1305-2_10.jpg

Pic legend10 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-beta tubulin antibody. Counter stained with hematoxylin.

Pic img11 :

https://storage.huabio.cn/huabio/productImg/M1305-2_11.jpg

Pic legend11 :

Flow cytometric analysis of HeLa cells labeling beta Tubulin. Cells were fixed and permeabilized. Then stained with the primary antibody (M1305-2, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img12 :

https://storage.huabio.cn/huabio/productImg/M1305-2_12.jpg

Pic legend12 :

Flow cytometric analysis of NIH/3T3 cells labeling beta Tubulin. Cells were fixed and permeabilized. Then stained with the primary antibody (M1305-2, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img13 :

https://storage.huabio.cn/huabio/productImg/M1305-2_13.jpg

Pic legend13 :

Immunocytochemistry analysis of PC-12 cells labeling beta Tubulin with Mouse anti-beta Tubulin antibody (M1305-2) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-beta Tubulin antibody (M1305-2) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

Pic img14 :

https://storage.huabio.cn/huabio/productImg/M1305-2_14.jpg

Pic legend14 :

Flow cytometric analysis of PC-12 HeLa cells labeling beta Tubulin. Cells were fixed and permeabilized. Then stained with the primary antibody (M1305-2, 1μg/mL) (red) compared with Mouse IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage