product summary
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company name :
HUABIO
product type :
antibody
product name :
CHD1
catalog :
M1211-5
quantity :
100μl
price :
360.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
2-2
reactivity :
human, mouse
application :
western blot, immunohistochemistry - paraffin section
more info or order :
HUABIO product webpage
image
image 1 :
HUABIO M1211-5 image 1
Western blot analysis of CHD1 on different lysates with Mouse anti-CHD1 antibody (M1211-5) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: MOLT-4 cell lysate Lane 4: COS-1 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 197 kDa Observed band size: 260 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1211-5) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
HUABIO M1211-5 image 2
Western blot analysis of CHD1 on different lysates with Mouse anti-CHD1 antibody (M1211-5) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-CHD1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 197 kDa Observed band size: 260 kDa Exposure time: 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1211-5) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
image 3 :
HUABIO M1211-5 image 3
Western blot analysis on recombinant protein using anti-CHD1 Mouse mAb (Cat. # M1211-5).
product information
SKU :
M1211-5
Target name :
CHD1
Species reactivity :
Human,Mouse
Applications :
WB,IHC-P
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within Human CHD1 aa 1,479-1,710 / 1,710.
Uniprot id :
O14646>SwissProt: O14646 Human;SwissProt: P40201 Mouse
Host :
Mouse
Clone number :
2-2
Isotype :
IgG1
Size :
100μl
List Price :
360.00 USD
Storage Buffer :
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Mouse monoclonal Antibody
Positive control :
HeLa cell lysate, HEK-293 cell lysate, MOLT-4 cell lysate, COS-1 cell lysate, mouse testis tissue.
Molecular wt :
Predicted band size: 197 kDa
Subcellular location :
Nucleus, Cytoplasm.
Concentration :
2 mg/mL.
Recommended dilutions :
WB: 1:1,000 ;IHC-P: 1:100
Advanced Validation :
Knockdown (KD)
Pic img4 :
https://storage.huabio.cn/huabio/productImg/M1211-5_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin- embedded mouse testis tissue using anti-CHD1 Mouse mAb (Cat. # M1211-5).
more info or order :
HUABIO product webpage
company information
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
headquarters: USA
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!