Anti-Beta actin Antibody [B4-B2] | HUABIO M1210-2TR product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Anti-Beta actin Antibody [B4-B2]

catalog :

M1210-2TR

quantity :

20 uL

price :

99 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

B4-B2

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

product information

SKU :

M1210-2TR

Target name :

Anti-Beta actin Antibody [B4-B2]

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within N-terminal of Human beta actin.

Uniprot id :

SwissProt: P60709 Human;SwissProt: P60710 Mouse;SwissProt: P60711 Rat

Host :

Mouse

Clone number :

B4-B2

Isotype :

IgG1

Size :

20 uL

List Price :

99 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Store at -20℃. Stable for 12 months from date of receipt.

Purity :

Immunogen affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

NIH/3T3, NIH/3T3 cell lysate, PC12 cell lysate, MCF-7 cell lysate, HepG2 cell lysate, Hela cell lysate, mouse lungtissue lysate, human colon carcinoma tissue, mouse kidney tissue.

Molecular wt :

Predicted band size: 42 kDa

Subcellular location :

Cytoplasm

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:10,000;IF-Cell: 1:100-1:200;IHC-P: 1:100-1:200;FC: 1:100-1:200

Pic img4 :

M1210-2_2.jpg

Pic img5 :

Western blot analysis of Beta actin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M1210-2, 1/10,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH/3T3 cell lysate Lane 2: PC-12 cell lysate Lane 3: MCF-7 cell lysate Lane 4: HepG2 cell lysate Lane 5: Hela cell lysate Lane 6: Mouse lung tissue lysate

Pic legend5 :

M1210-2_3.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Beta actin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1210-2, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

M1210-2_4.jpg

Pic img8 :

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Beta actin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1210-2, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage