Bcl-2 | HUABIO M1206-4 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Bcl-2

catalog :

M1206-4

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

F7-1-A1

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of BCL-2 on Jurkat cell lysate using anti-BCL-2 antibody at 1/100 dilution.

image 2 :

Western blot analysis of Bcl-2 on rat spleen tissue lysates with Mouse anti-Bcl-2 antibody (M1206-4) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1206-4) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunofluorescent staining of HepG2 cells using anti-Bcl-2 Mouse mAb (Cat. # M1206-4).

product information

SKU :

M1206-4

Target name :

Bcl-2

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human BCL-2 aa 1-200.

Uniprot id :

P10415>SwissProt: P10415 Human;SwissProt: P10417 Mouse;SwissProt: P49950 Rat

Host :

Mouse

Clone number :

F7-1-A1

Isotype :

IgG2b

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

Jurkat cell lysate, rat spleen tissue lysate, HepG2, human breast carcinoma tissue, mouse spleen tissue.

Molecular wt :

Predicted band size: 26 kDa

Subcellular location :

Mitochondrion outer membrane, Nucleus membrane, Endoplasmic reticulum membrane, Cytoplasm.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:500 ;IF-Cell: 1:500 ;IHC-P: 1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/M1206-4_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-BCL-2 antibody. Counter stained with hematoxylin.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/M1206-4_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-BCL-2 antibody. Counter stained with hematoxylin.

more info or order :

HUABIO product webpage