Alpha-cardiac actin | HUABIO M1206-1 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Alpha-cardiac actin

catalog :

M1206-1

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

33-32

reactivity :

human, mouse, rat, zebrafish

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Alpha-cardiac actin on different lysates with Mouse anti-Alpha-cardiac actin antibody (M1206-1) at 1/1,000 dilution. Lane 1: Mouse lung tissue lysate (40 µg/Lane) Lane 2: Mouse heart tissue lysate (40 µg/Lane) Lane 3: Rat heart tissue lysate (40 µg/Lane) Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1206-1) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human heart tissue with Mouse anti-Alpha-cardiac actin antibody (M1206-1) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1206-1) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Mouse anti-Alpha-cardiac actin antibody (M1206-1) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1206-1) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

M1206-1

Target name :

Alpha-cardiac actin

Species reactivity :

Human,Mouse,Rat,Zebrafish

Applications :

WB,IF-Cell,IHC-P,IF-Tissue,IHC-Fr

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human Alpha-cardiac actin aa 1-50 / 377.

Uniprot id :

P68032>SwissProt: P68032 Human;SwissProt: P68033 Mouse;SwissProt: P68035 Rat

Host :

Mouse

Clone number :

33-32

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

Mouse lung tissue lysate, mouse heart tissue lysate, rat heart tissue lysate, Hela, human heart tissue, mouse heart tissue, rat heart tissue.

Molecular wt :

Predicted band size: 42 kDa

Subcellular location :

Cytoplasm, cytoskeleton.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:50 ;IHC-P: 1:1,000 ;IF-Tissue: 1:200 ;IHC-Fr: 1:500

Pic img4 :

https://storage.huabio.cn/huabio/productImg/M1206-1_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat heart tissue with Mouse anti-Alpha-cardiac actin antibody (M1206-1) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1206-1) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/M1206-1_5.jpg

Pic legend5 :

Immunofluorescence analysis of paraffin-embedded human heart tissue labeling Alpha-cardiac actin with Mouse anti-Alpha-cardiac actin antibody (M1206-1) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (M1206-1, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Pic img6 :

https://storage.huabio.cn/huabio/productImg/M1206-1_6.jpg

Pic legend6 :

Application: IHC-Fr Species: Mouse Site: heart Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Pic img7 :

https://storage.huabio.cn/huabio/productImg/M1206-1_7.jpg

Pic legend7 :

Application: IHC-Fr Species: Rat Site: heart Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Pic img8 :

https://storage.huabio.cn/huabio/productImg/M1206-1_8.jpg

Pic legend8 :

Immunofluorescent staining of Hela cells using anti-Alpha-cardiac actin Mouse mAb (Cat. # M1206-1).

more info or order :

HUABIO product webpage