Histone H3 (tri methyl K9) | HUABIO M1112-3-50UL product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Histone H3 (tri methyl K9)

catalog :

M1112-3-50UL

quantity :

50μl

price :

205.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1-6

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Histone H3 (tri methyl K9) on different lysates with Mouse anti-Histone H3 (tri methyl K9) antibody (M1112-3) at 1/1,000 dilution. Lane 1: F9 cell lysate Lane 2: PC-12 cell lysate Lane 3: NCCIT cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: minute; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1112-3) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Tri-Methyl-Histone H3 (Lys9) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M1112-3, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane A: F9 cell lysate Lane B: F9+non-methyl peptide Lane C: F9+Tri-methl (lys9) peptide Lane D: PC12 cell lysate Lane E: NCCIT cell lysate

image 3 :

Immunocytochemistry analysis of HeLa cells labeling Histone H3 (tri methyl K9) with Mouse anti-Histone H3 (tri methyl K9) antibody (M1112-3) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Histone H3 (tri methyl K9) antibody (M1112-3) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

M1112-3-50UL

Target name :

Histone H3 (tri methyl K9)

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within N terminal human Histone H3 including tri methyl K9 conjugated to keyhole limpet haemocyanin.

Uniprot id :

P68431>SwissProt: P68431 Human;SwissProt: P68433 Mouse;SwissProt: Q6LED0 Rat

Host :

Mouse

Clone number :

1-6

Isotype :

IgG1

Size :

50μl

List Price :

205.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein G affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

F9 cell lysate, PC-12 cell lysate, NCCIT cell lysate, HeLa, NIH/3T3, human testis tissue, mouse testis tissue, mouse epididymis tissue, rat testis tissue.

Molecular wt :

Predicted band size: 15 kDa

Subcellular location :

Nucleus.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:5,000-1:10,000 ;IF-Cell: 1:250 ;IHC-P: 1:1,000 ;IF-Tissue: 1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/M1112-3_4.jpg

Pic legend4 :

Immunocytochemistry analysis of NIH/3T3 cells labeling Histone H3 (tri methyl K9) with Mouse anti-Histone H3 (tri methyl K9) antibody (M1112-3) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Histone H3 (tri methyl K9) antibody (M1112-3) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/M1112-3_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human testis tissue with Mouse anti-Histone H3 (tri methyl K9) antibody (M1112-3) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1112-3) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/M1112-3_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Mouse anti-Histone H3 (tri methyl K9) antibody (M1112-3) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1112-3) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/M1112-3_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded mouse epididymis tissue with Mouse anti-Histone H3 (tri methyl K9) antibody (M1112-3) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1112-3) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/M1112-3_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Tri-Methyl-Histone H3 (Lys9) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1112-3, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage