GRAMD1A | HUABIO M1010-3 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

GRAMD1A

catalog :

M1010-3

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1-E4

reactivity :

human, mouse

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of GRAMD1A on NIH/3T3 (1) and SHG-44 (2) cells lysates using anti-GRAMD1A antibody at 1/1,000 dilution.

image 2 :

Immunocytochemistry analysis of A549 cells labeling GRAMD1A with Mouse anti-GRAMD1A antibody (M1010-3) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-GRAMD1A antibody (M1010-3) at 1/50 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

image 3 :

Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-GRAMD1A antibody (M1010-3) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1010-3) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

M1010-3

Target name :

GRAMD1A

Species reactivity :

Human,Mouse

Applications :

WB,IF-Cell,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human GRAMD1A aa 1-724 / 724.

Uniprot id :

Q96CP6>SwissProt: Q96CP6 Human

Host :

Mouse

Clone number :

1-E4

Isotype :

IgM

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

NIH/3T3 cells lysates, SHG-44 cells lysates, A549, human liver tissue, human liver carcinoma tissue.

Molecular wt :

Predicted band size: 81 kDa

Subcellular location :

Endoplasmic reticulum membrane, Cell membrane, Cytoplasmic vesicle, autophagosome.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000;IF-Cell: 1:50-1:200;IHC-P: 1:50-1:400

Pic img4 :

https://storage.huabio.cn/huabio/productImg/M1010-3_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Mouse anti-GRAMD1A antibody (M1010-3) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1010-3) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage