CD80 | HUABIO M1007-10 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CD80

catalog :

M1007-10

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

8-E5

reactivity :

human, mouse

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CD80 on human brain tissue lysates with Mouse anti-CD80 antibody (M1007-10) at 1/1,000 dilution. Lysates/proteins at 40 µg/Lane. Predicted band size: 33 kDa Observed band size: 60 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1007-10) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin- embedded human brain tissue using using anti-CD80 Mouse mAb (Cat. # M1007-10).

image 3 :

Flow cytometric analysis of HUVEC (left, negative) and Raji (right, positive) cells labeling CD80. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (M1007-10, 1/1,000) (red) compared with Mouse IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

M1007-10

Target name :

CD80

Species reactivity :

Human,Mouse

Applications :

WB,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human CD80 aa 74-118 / 288.

Uniprot id :

P33681>SwissProt: P33681 Human;SwissProt: Q00609 Mouse

Host :

Mouse

Clone number :

8-E5

Isotype :

IgG2b

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

Human brain tissue lysates, human brain tissue, Raji.

Molecular wt :

Predicted band size: 33 kDa

Subcellular location :

Membrane

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:50 ;FC: 1:1,000

Advanced Validation :

Relative expression (RE)

more info or order :

HUABIO product webpage