antibody

Anti-FAM38A/PIEZO1 Antibody [2-10]

M1005-2TR

20 uL

99 USD

monoclonal

mouse

nonconjugated

2-10

human, mouse, rat

western blot, immunohistochemistry - paraffin section

Anti-FAM38A/PIEZO1 Antibody [2-10]

Human,Mouse,Rat

WB,IF-Cell,IHC-P

Non-conjugated

Recombinant protein within Human Protein FAM38A aa 1275-1540 / 2521.

SwissProt: Q92508 Human;SwissProt: E2JF22 Mouse;SwissProt: Q0KL00 Rat

Mouse

2-10

IgG2a

20 uL

99 USD

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Liquid

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Protein A affinity purified.

Mouse monoclonal Antibody

MCF-7, Hela, Siha, A431, mouse brain tissue, rat brain tissue, rat hippocampus tissue.

Predicted band size: 287 kD

Endoplasmic reticulum membrane. Cell membrane.

2 mg/mL.

WB: 1:500-1:2,000 ;IF-Cell: 1:100 ;IHC-P: 1:200-1:600

M1005-2_2.jpg

Western blot analysis of FAM38A/PIEZO1 on MCF-7 cell lysates with Mouse anti-FAM38A/PIEZO1 antibody (M1005-2) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 287 kDa Observed band size: 287 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1005-2) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

M1005-2_3.jpg

Immunocytochemistry analysis of Hela cells labeling FAM38A/PIEZO1 with Mouse anti-FAM38A/PIEZO1 antibody (M1005-2) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-FAM38A/PIEZO1 antibody (M1005-2) at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

M1005-2_4.jpg

Immunocytochemistry analysis of Siha cells labeling FAM38A/PIEZO1 with Mouse anti-FAM38A/PIEZO1 antibody (M1005-2) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-FAM38A/PIEZO1 antibody (M1005-2) at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

M1005-2_5.jpg

Immunocytochemistry analysis of A431 cells labeling FAM38A/PIEZO1 with Mouse anti-FAM38A/PIEZO1 antibody (M1005-2) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-FAM38A/PIEZO1 antibody (M1005-2) at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

M1005-2_6.jpg

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-FAM38A/PIEZO1 antibody (M1005-2) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1005-2) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

M1005-2_7.jpg

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-FAM38A/PIEZO1 antibody (M1005-2) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1005-2) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

M1005-2_8.jpg

Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Mouse anti-FAM38A/PIEZO1 antibody (M1005-2) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1005-2) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.