product summary
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company name :
HUABIO
product type :
antibody
product name :
FAM38A / PIEZO1
catalog :
M1005-2
quantity :
100μl
price :
360.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
2-10
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry - paraffin section
more info or order :
product information
SKU :
M1005-2
Target name :
FAM38A / PIEZO1
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IF-Cell,IHC-P
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within Human PIEZO1 aa 1275-1540 / 2521.
Uniprot id :
Q92508>SwissProt: Q92508 Human;SwissProt: E2JF22 Mouse;SwissProt: Q0KL00 Rat
Host :
Mouse
Clone number :
2-10
Isotype :
IgG2a
Size :
100μl
List Price :
360.00 USD
Storage Buffer :
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Mouse monoclonal Antibody
Positive control :
MCF-7, Hela, Siha, A431, mouse brain tissue, rat brain tissue, rat hippocampus tissue.
Molecular wt :
Predicted band size: 287 kD
Subcellular location :
Endoplasmic reticulum membrane. Cell membrane.
Concentration :
2 mg/mL.
Recommended dilutions :
WB: 1:500-1:2,000
;IF-Cell: 1:100
;IHC-P: 1:200-1:600
Pic img4 :
https://storage.huabio.cn/huabio/productImg/M1005-2_4.jpg
Pic legend4 :
Immunocytochemistry analysis of Siha cells labeling FAM38A / PIEZO1 with Mouse anti-FAM38A / PIEZO1 antibody (M1005-2) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-FAM38A / PIEZO1 antibody (M1005-2) at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/M1005-2_5.jpg
Pic legend5 :
Immunocytochemistry analysis of A431 cells labeling FAM38A / PIEZO1 with Mouse anti-FAM38A / PIEZO1 antibody (M1005-2) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-FAM38A / PIEZO1 antibody (M1005-2) at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/M1005-2_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-FAM38A / PIEZO1 antibody (M1005-2) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1005-2) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/M1005-2_7.jpg
Pic legend7 :
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-FAM38A / PIEZO1 antibody (M1005-2) at 1/600 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1005-2) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img8 :
https://storage.huabio.cn/huabio/productImg/M1005-2_8.jpg
Pic legend8 :
Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Mouse anti-FAM38A / PIEZO1 antibody (M1005-2) at 1/600 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1005-2) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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