product summary
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company name :
HUABIO
product type :
antibody
product name :
6X His tag
catalog :
M0812-3
quantity :
100μl
price :
360.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
8-F0
application :
western blot, immunoprecipitation
more info or order :
image
image 1 :
Western blot analysis of 6X His tag on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M0812-3, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: C-terminal His-tagged recombinant protein
Lane 2: N-terminal His-tagged recombinant protein
image 2 :
His tag was immunoprecipitated in 2µg C terminal His Tag fusion protein lysate with M0812-3 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using R1207-2 at 1/500 dilution. Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 60 mins at room temperature.
Lane 1: His Tag fusion protein lysate (input).
Lane 2: M0812-3 IP in His Tag fusion protein lysate.
Lane 3: Mouse IgG instead of M0812-3 in His Tag fusion protein lysate.
Blocking/Dilution buffer: 5% NFDM/TBST
product information
SKU :
M0812-3
Target name :
6X His tag
Species reactivity :
Species independent
Applications :
WB,IP
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide immune sequence is CHHHHHHHH.
Uniprot id :
>
Host :
Mouse
Clone number :
8-F0
Isotype :
IgG2a
Size :
100μl
List Price :
360.00 USD
Storage Buffer :
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein G affinity purified.
Product type :
Mouse monoclonal Antibody
Positive control :
C-terminal His-tagged recombinant protein.
Concentration :
2 mg/mL.
Recommended dilutions :
WB: 1:2,000
;IP: 2-5 µg/ml.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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