Beta III Tubulin | HUABIO M0805-8 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Beta III Tubulin

catalog :

M0805-8

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A8-D10

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Beta III Tubulin on different lysates with Mouse anti-Beta III Tubulin antibody (M0805-8) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: U-87 MG cell lysate Lane 3: A-172 cell lysate Lane 4: Neuro-2a cell lysate Lane 5: PC-12 cell lysate Lane 6: Mouse brain tissue lysate Lane 7: Rat brain tissue lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 11 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M0805-8) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% BSA at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of HEK-293 cells labeling Beta III Tubulin with Mouse anti-Beta III Tubulin antibody (M0805-8) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Beta III Tubulin antibody (M0805-8) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunocytochemistry analysis of SH-SY5Y cells labeling Beta III Tubulin with Mouse anti-Beta III Tubulin antibody (M0805-8) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Beta III Tubulin antibody (M0805-8) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

M0805-8

Target name :

Beta III Tubulin

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,FC,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide (KLH-coupled) within human Tubulin beta-3 chain aa 401-450.

Uniprot id :

Q13509>SwissProt: Q13509 Human;SwissProt: Q9ERD7 Mouse;SwissProt: Q4QRB4 Rat

Host :

Mouse

Clone number :

A8-D10

Isotype :

IgG2a

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

SH-SY5Y cell lysate, U-87 MG cell lysate, A-172 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, HEK-293, SH-SY5Y, PC-12, Neuro-2a, human brain tissue, mouse brain tissue, rat brain tissue, mouse hippocampus tissue, MCF7.

Molecular wt :

Predicted band size: 50 kDa

Subcellular location :

Cytoplasm. Cytoskeleton. Microtubule.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:2,000-1:5,000 ;IF-Cell: 1:500-1:1,000 ;IHC-P: 1:2,000 ;FC: 1:1,000 ;IF-Tissue: 1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/M0805-8_4.jpg

Pic legend4 :

Immunocytochemistry analysis of PC-12 cells labeling Beta III Tubulin with Mouse anti-Beta III Tubulin antibody (M0805-8) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Beta III Tubulin antibody (M0805-8) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/M0805-8_5.jpg

Pic legend5 :

Immunocytochemistry analysis of Neuro-2a cells labeling Beta III Tubulin with Mouse anti-Beta III Tubulin antibody (M0805-8) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Beta III Tubulin antibody (M0805-8) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/M0805-8_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-Beta III Tubulin antibody (M0805-8) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M0805-8) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/M0805-8_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-Beta III Tubulin antibody (M0805-8) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M0805-8) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/M0805-8_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-Beta III Tubulin antibody (M0805-8) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M0805-8) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/M0805-8_9.jpg

Pic legend9 :

Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling Beta III Tubulin (M0805-8) and Synaptophysin (ET1606-56). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Beta III Tubulin (M0805-8, green) at 1/200 dilution and Synaptophysin (ET1606-56, red) at 1/200 dilution overnight at 4 ℃, washed with PBS. Alexa Fluor® 488 conjugate-Goat anti-Mouse IgG and Alexa Fluor® 594 conjugate-Goat anti-Rabbit IgG were used as the secondary antibodies at 1/500 dilution. DAPI was used as nuclear counterstain.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/M0805-8_10.jpg

Pic legend10 :

Immunofluorescence analysis of paraffin-embedded mouse hippocampus tissue labeling Beta III Tubulin (M0805-8) and Synaptophysin (ET1606-56). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Beta III Tubulin (M0805-8, green) at 1/200 dilution and Synaptophysin (ET1606-56, red) at 1/200 dilution overnight at 4 ℃, washed with PBS. Alexa Fluor® 488 conjugate-Goat anti-Mouse IgG and Alexa Fluor® 594 conjugate-Goat anti-Rabbit IgG were used as the secondary antibodies at 1/500 dilution. DAPI was used as nuclear counterstain.

Pic img11 :

https://storage.huabio.cn/huabio/productImg/M0805-8_11.jpg

Pic legend11 :

Immunofluorescence analysis of paraffin-embedded rat brain tissue labeling Beta III Tubulin (M0805-8) and Synaptophysin (ET1606-56). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Beta III Tubulin (M0805-8, green) at 1/200 dilution and Synaptophysin (ET1606-56, red) at 1/200 dilution overnight at 4 ℃, washed with PBS. Alexa Fluor® 488 conjugate-Goat anti-Mouse IgG and Alexa Fluor® 594 conjugate-Goat anti-Rabbit IgG were used as the secondary antibodies at 1/500 dilution. DAPI was used as nuclear counterstain.

Pic img12 :

https://storage.huabio.cn/huabio/productImg/M0805-8_12.jpg

Pic legend12 :

Flow cytometric analysis of MCF7 cells labeling Beta III Tubulin. Cells were fixed and permeabilized. Then stained with the primary antibody (M0805-8, 1/1,000) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage