14-3-3 alpha+beta | HUABIO M0407-14 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

14-3-3 alpha+beta

catalog :

M0407-14

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

9-26

reactivity :

human, mouse, rat, zebrafish

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of 14-3-3 alpha+beta on different lysates with Mouse anti-14-3-3 alpha+beta antibody (M0407-14) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-14-3-3 alpha+beta KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M0407-14) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of 14-3-3 alpha+beta on A549 cell lysates with Mouse anti-14-3-3 alpha+beta antibody (M0407-14) at 1/5,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M0407-14) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

image 3 :

Western blot analysis on mouse liver tissue lysates using anti-14-3-3b/a Mouse mAb (Cat. # M0407-14).

product information

SKU :

M0407-14

Target name :

14-3-3 alpha+beta

Species reactivity :

Human,Mouse,Zebrafish,Rat

Applications :

WB,IF-Cell,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human 14-3-3 b / a aa 1-246 / 246.

Uniprot id :

P31946>SwissProt: P31946 Human

Host :

Mouse

Clone number :

9-26

Isotype :

IgG2a

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

A549 cell lysates, mouse liver tissue lysates, hybrid fish (crucian-carp) brain tissue lysates, 293T, human breast carcinoma tissue, Zebrafish tissue.

Molecular wt :

Predicted band size: 28 kDa

Subcellular location :

Cytoplasm, Membrane, Vacuole.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:5,000 ;IF-Cell: 1:50-1:100 ;IHC-P: 1:200

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/M0407-14_4.jpg

Pic legend4 :

Western blot analysis of 14-3-3b/a on hybrid fish (crucian-carp) brain tissue lysate using anti-14-3-3b/a antibody at 1/500 dilution.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/M0407-14_5.jpg

Pic legend5 :

Immunocytochemistry analysis of 293T cells labeling 14-3-3 alpha+beta with Mouse anti-14-3-3 alpha+beta antibody (M0407-14) at 1/50 dilution.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/M0407-14_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using anti-14-3-3b/a Mouse mAb (Cat. # M0407-14).

Pic img7 :

https://storage.huabio.cn/huabio/productImg/M0407-14_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded Zebrafish tissue using anti-14-3-3b/a antibody. Counter stained with hematoxylin.

more info or order :

HUABIO product webpage