GABRA2 | HUABIO HA751620 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

GABRA2

catalog :

HA751620

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH17-28

reactivity :

mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Mouse Site: hippocampus Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

image 2 :

Application: IHC-Fr Species: Rat Site: hippocampus Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-GABRA2 antibody (HA751620) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751620) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA751620

Target name :

GABRA2

Species reactivity :

Mouse,Rat

Applications :

IHC-Fr,IHC-P,WB,IP

Conjugate :

Non-conjugated

Uniprot id :

P26048>SwissProt: P26048 Mouse;SwissProt: P23576 Rat

Host :

Rabbit

Clone number :

PSH17-28

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Mouse hippocampus tissue, rat hippocampus tissue, Mouse brain tissue lysate, Rat cerebellum tissue lysate.

Molecular wt :

Predicted band size: 51 kDa

Subcellular location :

Postsynaptic cell membrane, Cell membrane, Cytoplasmic vesicle membrane, Cell projection, dendrite.

Concentration :

1 mg/mL.

Recommended dilutions :

IHC-Fr: 1:500 ;IHC-P: 1:200-1:1,000 ;WB: 1:2,000 ;IP: 1-2μg/sample

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA751620_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-GABRA2 antibody (HA751620) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751620) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA751620_5.jpg

Pic legend5 :

Western blot analysis of GABRA2 on different lysates with Rabbit anti-GABRA2 antibody (HA751620) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat cerebellum tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 51 kDa Observed band size: 51 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751620) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA751620_6.jpg

Pic legend6 :

GABRA2 was immunoprecipitated from 0.2 mg mouse brain tissue lysate with HA751620 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751620 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: mouse brain tissue lysate (input) Lane 2: HA751620 IP in mouse brain tissue lysate Lane 3: Rabbit IgG instead of HA751620 in mouse brain tissue lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 10 seconds; ECL: K1801

more info or order :

HUABIO product webpage