Neuropeptide Y | HUABIO HA751601 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Neuropeptide Y

catalog :

HA751601

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH16-52

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Mouse Site: brain Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

image 2 :

Application: IHC-Fr Species: Rat Site: brain Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

image 3 :

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue with Rabbit anti-Neuropeptide Y antibody (HA751601) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751601) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA751601

Target name :

Neuropeptide Y

Species reactivity :

Human,Mouse,Rat

Applications :

IHC-Fr,IHC-P,WB

Conjugate :

Non-conjugated

Uniprot id :

P01303>SwissProt: P01303 Human;SwissProt: P57774 Mouse;SwissProt: P07808 Rat

Host :

Rabbit

Clone number :

PSH16-52

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Human cerebrum tissue, mouse cerebrum tissue, rat cerebrum tissue, SH-SY5Y treated with 200nM TPA for 24 hours cell lysate, HEK-293 transfected with Neuropeptide Y then treated with 200nM TPA for 24 hours cell lysate.

Molecular wt :

Predicted band size: 11 kDa

Subcellular location :

Secreted, Cytoplasmic vesicle, secretory vesicle, neuronal dense core vesicle.

Concentration :

1 mg/mL.

Recommended dilutions :

IHC-Fr: 1:500 ;IHC-P: 1:1,000 ;WB: 1:2,000

Advanced Validation :

Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA751601_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue with Rabbit anti-Neuropeptide Y antibody (HA751601) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751601) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA751601_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue with Rabbit anti-Neuropeptide Y antibody (HA751601) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751601) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA751601_6.jpg

Pic legend6 :

Western blot analysis of Neuropeptide Y on different lysates with Rabbit anti-Neuropeptide Y antibody (HA751601) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM TPA for 24 hours cell lysate Lane 3: HEK-293 transfected with Neuropeptide Y cell lysate Lane 4: HEK-293 transfected with Neuropeptide Y, then treated with 200nM TPA for 24 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 11 kDa Observed band size: 15 kDa Exposure time: Lane 1-2: 59 seconds; Lane 3-4: 10 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751601) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

more info or order :

HUABIO product webpage