product summary
Loading...
company name :
HUABIO
product type :
antibody
product name :
CIP2A
catalog :
HA751590
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH16-12
reactivity :
human, mouse, rat
application :
western blot, immunoprecipitation
more info or order :
image
image 1 :
Western blot analysis of CIP2A on different lysates with Rabbit anti-CIP2A antibody (HA751590) at 1/5,000 dilution.
Lane 1: RD cell lysate (20 µg/Lane)
Lane 2: MG-63 cell lysate (20 µg/Lane)
Lane 3: Jurkat cell lysate (20 µg/Lane)
Lane 4: HEK-293 cell lysate (20 µg/Lane)
Lane 5: Hela cell lysate (20 µg/Lane)
Lane 6: NIH-3T3 cell lysate (20 µg/Lane)
Lane 7: C6 cell lysate (20 µg/Lane)
Predicted band size: 102 kDa
Observed band size: 102 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751590) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
CIP2A was immunoprecipitated from 0.2 mg Jurkat cell lysate with HA751590 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751590 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Jurkat cell lysate (input)
Lane 2: HA751590 IP in Jurkat cell lysate
Lane 3: Rabbit IgG instead of HA751590 in Jurkat cell lysate
Blocking/Dilution buffer: primary antibody dilution (K1803)
Exposure time: 2 minutes 6 seconds; ECL: K1801
product information
SKU :
HA751590
Target name :
CIP2A
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IP
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within PEP240387 220-280
Uniprot id :
Q8TCG1>SwissProt: Q8TCG1 Human;SwissProt: Q8BWY9 Mouse;Entrez Gene: 360711 Rat
Host :
Rabbit
Clone number :
PSH16-12
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
RD cell lysate, MG-63 cell lysate, Jurkat cell lysate, HEK-293 cell lysate, Hela cell lysate, NIH-3T3 cell lysate, C6 cell lysate.
Molecular wt :
Predicted band size: 102 kDa
Subcellular location :
Cytoplasm, Chromosome.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:5,000
;IP: 1:5,000
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
related products
browse more products
questions and comments