Dishevelled 3 | HUABIO HA751584 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Dishevelled 3

catalog :

HA751584

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH15-93

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Dishevelled 3 on different lysates with Rabbit anti-Dishevelled 3 antibody (HA751584) at 1/5,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: NIH/3T3 cell lysate (20 µg/Lane) Lane 3: C6 cell lysate (20 µg/Lane) Lane 4: COS-1 cell lysate (20 µg/Lane) Lane 5: Mouse brain tissue lysate (40 µg/Lane) Lane 6: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 78 kDa Observed band size: 78 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751584) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Dishevelled 3 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA751584 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751584 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA751584 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA751584 in HeLa cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 25 seconds; ECL: K1801

product information

SKU :

HA751584

Target name :

Dishevelled 3

Species reactivity :

Human,Mouse,Rat,Monkey

Applications :

WB,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human Dishevelled 3 aa 621-670.

Uniprot id :

Q92997>SwissProt: Q92997 Human;SwissProt: Q61062 Mouse;Entrez Gene: 303811 Rat

Host :

Rabbit

Clone number :

PSH15-93

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, NIH/3T3 cell lysate, C6 cell lysate, COS-1 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate.

Molecular wt :

Predicted band size: 78 kDa

Subcellular location :

Cytoplasm

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IP: 1-2μg/sample

more info or order :

HUABIO product webpage