SIRT5 | HUABIO HA751578 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

SIRT5

catalog :

HA751578

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH15-71

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of SIRT5 on different lysates with Rabbit anti-SIRT5 antibody (HA751578) at 1/2,000 dilution. Lane 1: 293T cell lysate (20 µg/Lane) Lane 2: Hepa-1-6 cell lysate (20 µg/Lane) Lane 3: RAW264.7 cell lysate (20 µg/Lane) Lane 4: C6 cell lysate (20 µg/Lane) Lane 5: Rat liver tissue lysate (20 µg/Lane) Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 1 minute 50 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751578) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-SIRT5 antibody (HA751578) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751578) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA751578

Target name :

SIRT5

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Uniprot id :

Q9NXA8>SwissProt: Q9NXA8 Human;SwissProt: Q8K2C6 Mouse;SwissProt: Q68FX9 Rat

Host :

Rabbit

Clone number :

PSH15-71

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

293T cell lysate, Hepa-1-6 cell lysate, RAW264.7 cell lysate, C6 cell lysate, Rat liver tissue lysate, human liver tissue.

Molecular wt :

Predicted band size: 34 kDa

Subcellular location :

Mitochondrion matrix, Mitochondrion intermembrane space, Cytoplasm, cytosol, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IHC-P: 1:1,000

more info or order :

HUABIO product webpage