product summary
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company name :
HUABIO
product type :
antibody
product name :
Acid sphingomyelinase
catalog :
HA751572
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH15-61
reactivity :
human, mouse, rat
application :
western blot, immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of Acid sphingomyelinase on different lysates with Rabbit anti-Acid sphingomyelinase antibody (HA751572) at 1/5,000 dilution.
Lane 1: THP-1 cell lysate (20 µg/Lane)
Lane 2: THP-1 treated with 80nM TPA overnight then treated with 100ng/mL LPS for 6 hours add 300n/mL BFA for 3 hours cell lysate (20 µg/Lane)
Lane 3: HepG2 cell lysate (20 µg/Lane)
Lane 4: HepG2 treated with 10ng/mL BFA for 1.5 hours cell lysate (20 µg/Lane)
Lane 5: MOLT-4 cell lysate (low expression) (20 µg/Lane)
Lane 6: COS-1 cell lysate (20 µg/Lane)
Predicted band size: 70 kDa
Observed band size: 70 kDa
Exposure time: 25 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751572) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of Acid sphingomyelinase on different lysates with Rabbit anti-Acid sphingomyelinase antibody (HA751572) at 1/5,000 dilution.
Lane 1: PC-12 cell lysate (40 µg/Lane)
Lane 2: PC-12 starved for 16 hours then treated with 200nM TPA for 4 hours cell lysate (40 µg/Lane)
Predicted band size: 70 kDa
Observed band size: 70 kDa
Exposure time: 1 minute 18 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751572) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 3 :
Western blot analysis of Acid sphingomyelinase on RAW264.7 cell lysates with Rabbit anti-Acid sphingomyelinase antibody (HA751572) at 1/2,000 dilution.
Lysates/proteins at 30 µg/Lane.
Predicted band size: 70 kDa
Observed band size: 70 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751572) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
product information
SKU :
HA751572
Target name :
Acid sphingomyelinase
Species reactivity :
Human,Mouse,Rat,Monkey
Applications :
WB,IHC-P,IP
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human Acid sphingomyelinase aa 47-631.
Uniprot id :
P17405>SwissProt: P17405 Human;SwissProt: Q04519 Mouse;Entrez Gene: 308909 Rat
Host :
Rabbit
Clone number :
PSH15-61
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
THP-1 treated with 80nM TPA overnight then treated with 100ng/mL LPS for 6 hours add 300n/mL BFA for 3 hours cell lysate, HepG2 cell lysate, HepG2 treated with 10ng/mL BFA for 1.5 hours cell lysate, COS-1 cell lysate, PC-12 cell lysate, PC-12 starved for 16 hours then treated with 200nM TPA for 4 hours cell lysate, RAW264.7 cell lysates, human kidney tissue, human liver tissue, mouse kidney tissue, mouse liver tissue, rat kidney tissue, rat liver tissue.
Molecular wt :
Predicted band size: 70 kDa
Subcellular location :
Lysosome, Lipid droplet, Secreted.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:2,000-1:5,000
;IHC-P: 1:100-1:200
;IP: 1-2μg/sample
Advanced Validation :
Cell treatment (CT)
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA751572_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Acid sphingomyelinase antibody (HA751572) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751572) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA751572_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Acid sphingomyelinase antibody (HA751572) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751572) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/HA751572_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Acid sphingomyelinase antibody (HA751572) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751572) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/HA751572_7.jpg
Pic legend7 :
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Acid sphingomyelinase antibody (HA751572) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751572) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img8 :
https://storage.huabio.cn/huabio/productImg/HA751572_8.jpg
Pic legend8 :
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Acid sphingomyelinase antibody (HA751572) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751572) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img9 :
https://storage.huabio.cn/huabio/productImg/HA751572_9.jpg
Pic legend9 :
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Acid sphingomyelinase antibody (HA751572) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751572) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img10 :
https://storage.huabio.cn/huabio/productImg/HA751572_10.jpg
Pic legend10 :
Acid sphingomyelinase was immunoprecipitated from 0.2 mg HepG2 cell lysate with HA751572 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751572 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HepG2 cell lysate (input)
Lane 2: HA751572 IP in HepG2 cell lysate
Lane 3: Rabbit IgG instead of HA751572 in HepG2 cell lysate
Blocking/Dilution buffer: primary antibody dilution (K1803)
Exposure time: 46 seconds; ECL: K1801
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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