TAZ / WWTR1 | HUABIO HA751569 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

TAZ / WWTR1

catalog :

HA751569

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH15-58

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of TAZ / WWTR1 on different lysates with Rabbit anti-TAZ / WWTR1 antibody (HA751569) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: PANC-1 cell lysate Lane 3: SH-SY5Y cell lysate (low expression) Lane 4: SK-MEL-28 cell lysate Lane 5: Raji cell lysate (negative) Lane 6: A375 cell lysate Lane 7: F9 cell lysate Lane 8: C2C12 cell lysate Lane 9: NIH/3T3 cell lysate Lane 10: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 44 kDa Observed band size: 50 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751569) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-TAZ / WWTR1 antibody (HA751569) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751569) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-TAZ / WWTR1 antibody (HA751569) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751569) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA751569

Target name :

TAZ / WWTR1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human TAZ / WWTR1 aa 1-400.

Uniprot id :

Q9GZV5>SwissProt: Q9GZV5 Human;SwissProt: Q9EPK5 Mouse;Entrez Gene: 295062 Rat

Host :

Rabbit

Clone number :

PSH15-58

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, PANC-1 cell lysate, SK-MEL-28 cell lysate, A375 cell lysate, F9 cell lysate, C2C12 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, human breast carcinoma tissue, human kidney tissue, human stomach tissue, mouse kidney tissue, mouse stomach tissue, rat kidney tissue, rat stomach tissue.

Molecular wt :

Predicted band size: 44 kDa

Subcellular location :

Nucleus, Cytoplasm, Cell membrane, Cell junction, tight junction.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IHC-P: 1:200-1:1,000 ;IP: 1-2μg/sample

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA751569_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-TAZ / WWTR1 antibody (HA751569) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751569) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA751569_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-TAZ / WWTR1 antibody (HA751569) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751569) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA751569_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue with Rabbit anti-TAZ / WWTR1 antibody (HA751569) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751569) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA751569_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-TAZ / WWTR1 antibody (HA751569) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751569) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA751569_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded rat stomach tissue with Rabbit anti-TAZ / WWTR1 antibody (HA751569) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751569) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA751569_9.jpg

Pic legend9 :

TAZ / WWTR1 was immunoprecipitated from 0.2 mg PANC-1 cell lysate with HA751569 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751569 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: PANC-1 cell lysate (input) Lane 2: HA751569 IP in PANC-1 cell lysate Lane 3: Rabbit IgG instead of HA751569 in PANC-1 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 3 minutes; ECL: K1801

Pic img10 :

https://storage.huabio.cn/huabio/productImg/HA751569_10.jpg

Pic legend10 :

TAZ / WWTR1 was immunoprecipitated from 0.2 mg C2C12 cell lysate with HA751569 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751569 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: C2C12 cell lysate (input) Lane 2: HA751569 IP in C2C12 cell lysate Lane 3: Rabbit IgG instead of HA751569 in C2C12 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 20 seconds; ECL: K1801

more info or order :

HUABIO product webpage