TAZ / WWTR1 | HUABIO HA751568 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

TAZ / WWTR1

catalog :

HA751568

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH15-57

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of TAZ / WWTR1 on different lysates with Rabbit anti-TAZ / WWTR1 antibody (HA751568) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: PANC-1 cell lysate Lane 3: SH-SY5Y cell lysate (low expression) Lane 4: SK-MEL-28 cell lysate Lane 5: Raji cell lysate (negative) Lane 6: A375 cell lysate Lane 7: COS-1 cell lysate Lane 8: F9 cell lysate Lane 9: C2C12 cell lysate Lane 10: NIH/3T3 cell lysate Lane 11: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 44 kDa Observed band size: 50 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751568) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

TAZ / WWTR1 was immunoprecipitated from 0.2 mg PANC-1 cell lysate with HA751568 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751568 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: PANC-1 cell lysate (input) Lane 2: HA751568 IP in PANC-1 cell lysate Lane 3: Rabbit IgG instead of HA751568 in PANC-1 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 1 minute 18 seconds; ECL: K1801

image 3 :

TAZ / WWTR1 was immunoprecipitated from 0.2 mg C2C12 cell lysate with HA751568 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751568 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: C2C12 cell lysate (input) Lane 2: HA751568 IP in C2C12 cell lysate Lane 3: Rabbit IgG instead of HA751568 in C2C12 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 10 seconds; ECL: K1801

product information

SKU :

HA751568

Target name :

TAZ / WWTR1

Species reactivity :

Human,Mouse,Rat,Monkey

Applications :

WB,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human TAZ / WWTR1 aa 1-400.

Uniprot id :

Q9GZV5>SwissProt: Q9GZV5 Human;SwissProt: Q9EPK5 Mouse;Entrez Gene: 295062 Rat

Host :

Rabbit

Clone number :

PSH15-57

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, PANC-1 cell lysate, SK-MEL-28 cell lysate, A375 cell lysate, COS-1 cell lysate, F9 cell lysate, C2C12 cell lysate, NIH/3T3 cell lysate, C6 cell lysate.

Molecular wt :

Predicted band size: 44 kDa

Subcellular location :

Nucleus, Cytoplasm, Cell membrane, Cell junction, tight junction.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IP: 1-2μg/sample

more info or order :

HUABIO product webpage