Ryanodine receptor 2 / RYR-2 | HUABIO HA751567 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Ryanodine receptor 2 / RYR-2

catalog :

HA751567

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH15-54

reactivity :

mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Ryanodine receptor 2 / RYR-2 on different lysates with Rabbit anti-Ryanodine receptor 2 / RYR-2 antibody (HA751567) at 1/2,000 dilution. Lane 1: Mouse heart tissue lysate Lane 2: Mouse skeletal muscle tissue lysate (negative) Lane 3: Mouse liver tissue lysate (negative) Lane 4: Rat heart tissue lysate Lane 5: Rat skeletal muscle tissue lysate (negative) Lane 6: Rat liver tissue lysate (negative) Lysates/proteins at 40 µg/Lane. Predicted band size: 565 kDa Observed band size: 565 kDa Exposure time: 1 minute 7 seconds; ECL: K1802; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751567) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-Ryanodine receptor 2 / RYR-2 antibody (HA751567) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751567) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse liver tissue (negative) with Rabbit anti-Ryanodine receptor 2 / RYR-2 antibody (HA751567) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751567) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA751567

Target name :

Ryanodine receptor 2 / RYR-2

Species reactivity :

Mouse,Rat

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human RYR-2 aa 1,301-1,600.

Uniprot id :

E9Q401>SwissProt: E9Q401 Mouse;SwissProt: B0LPN4 Rat

Host :

Rabbit

Clone number :

PSH15-54

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Mouse heart tissue lysate, Rat heart tissue lysate, mouse heart tissue.

Molecular wt :

Predicted band size: 565 kDa

Subcellular location :

Sarcoplasmic reticulum membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IHC-P: 1:200

Advanced Validation :

Relative expression (RE)

more info or order :

HUABIO product webpage