product summary
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company name :
HUABIO
product type :
antibody
product name :
Choline Acetyltransferase
catalog :
HA751564
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH15-49
reactivity :
human, mouse, rat
application :
immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Immunohistochemical analysis of paraffin-embedded mouse brain (habenular nucleus) tissue with Rabbit anti-Choline Acetyltransferase antibody (HA751564) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751564) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
image 2 :
Immunohistochemical analysis of paraffin-embedded mouse brain (caudate nucleus) tissue with Rabbit anti-Choline Acetyltransferase antibody (HA751564) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751564) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
image 3 :
Immunohistochemical analysis of paraffin-embedded mouse hindbrain tissue with Rabbit anti-Choline Acetyltransferase antibody (HA751564) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751564) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
HA751564
Target name :
Choline Acetyltransferase
Species reactivity :
Human,Mouse,Rat
Applications :
IHC-P
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within mouse Choline Acetyltransferase aa 1-641.
Uniprot id :
P28329>SwissProt: P28329 Human;SwissProt: Q03059 Mouse;SwissProt: P32738 Rat
Host :
Rabbit
Clone number :
PSH15-49
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
Mouse brain (habenular nucleus) tissue, mouse brain (caudate nucleus) tissue, mouse hindbrain tissue, rat brain (habenular nucleus) tissue, rat brain (caudate nucleus) tissue, rat hindbrain tissue.
Molecular wt :
Predicted band size: 72 kDa
Subcellular location :
Cytosol, nucleus, cytoplasm, neuron projection, presynapse.
Concentration :
1 mg/mL.
Recommended dilutions :
IHC-P: 1:200-1:1,000
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA751564_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded rat brain (habenular nucleus) tissue with Rabbit anti-Choline Acetyltransferase antibody (HA751564) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751564) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA751564_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded rat brain (caudate nucleus) tissue with Rabbit anti-Choline Acetyltransferase antibody (HA751564) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751564) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/HA751564_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded rat hindbrain tissue with Rabbit anti-Choline Acetyltransferase antibody (HA751564) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751564) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/HA751564_7.jpg
Pic legend7 :
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Choline Acetyltransferase antibody (HA751564) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751564) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img8 :
https://storage.huabio.cn/huabio/productImg/HA751564_8.jpg
Pic legend8 :
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-Choline Acetyltransferase antibody (HA751564) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751564) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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