product summary
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company name :
HUABIO
product type :
antibody
product name :
p16INK4a
catalog :
HA751555
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH15-40
reactivity :
mouse
application :
western blot, immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of p16INK4a on different lysates with Rabbit anti-p16INK4a antibody (HA751555) at 1/2,000 dilution.
Lane 1: A20 cell lysate
Lane 2: NIH/3T3 cell lysate (negative)
Lane 3: MEF cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 18 kDa
Observed band size: 16 kDa
Exposure time: 1 minute 50 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751555) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-p16INK4a antibody (HA751555) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751555) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
image 3 :
p16INK4a was immunoprecipitated from 0.2 mg MEF cell lysate with HA751555 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751555 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: MEF cell lysate (input)
Lane 2: HA751555 IP in MEF cell lysate
Lane 3: Rabbit IgG instead of HA751555 in MEF cell lysate
Blocking/Dilution buffer: primary antibody dilution (K1803)
Exposure time: 3 minutes; ECL: K1801
product information
SKU :
HA751555
Target name :
p16INK4a
Species reactivity :
Mouse
Applications :
WB,IHC-P,IP
Conjugate :
Non-conjugated
Uniprot id :
P51480>SwissProt: P51480 Mouse
Host :
Rabbit
Clone number :
PSH15-40
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
A20 cell lysate, MEF cell lysate, mouse spleen tissue.
Molecular wt :
Predicted band size: 18 kDa
Subcellular location :
Cytoplasm, Nucleus.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:2,000
;IHC-P: 1:200
;IP: 1:1,000
Advanced Validation :
Relative expression (RE)
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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