product summary
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company name :
HUABIO
product type :
antibody
product name :
Choline Acetyltransferase
catalog :
HA751543
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH15-05
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry - frozen section
more info or order :
HUABIO product webpage
image
image 1 :
HUABIO HA751543 image 1
Western blot analysis of Choline Acetyltransferase on different lysates with Rabbit anti-Choline Acetyltransferase antibody (HA751543) at 1/5,000 dilution. Lane 1: SH-SY5Y cell lysate (20 µg/Lane) Lane 2: Neuro-2a cell lysate (negative) (20 µg/Lane) Lane 3: Mouse brain tissue lysate (40 µg/Lane) Lane 4: Mouse cerebellum tissue lysate (40 µg/Lane) Lane 5: Rat brain tissue lysate (40 µg/Lane) Lane 6: Rat cerebellum tissue lysate (40 µg/Lane) Predicted band size: 83 kDa Observed band size: 50/75 kDa Exposure time: 3 minutess; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751543) at 1/5,000 dilution was used in primary antibody dilution (K1803) at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
HUABIO HA751543 image 2
Application: IHC-Fr Species: Mouse Site: brain Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required
image 3 :
HUABIO HA751543 image 3
Application: IHC-Fr Species: Rat Site: brain Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required
product information
SKU :
HA751543
Target name :
Choline Acetyltransferase
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IHC-Fr
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within mouse Choline Acetyltransferase aa 1-641.
Uniprot id :
P28329>SwissProt: P28329 Human;SwissProt: Q03059 Mouse;SwissProt: P32738 Rat
Host :
Rabbit
Clone number :
PSH15-05
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
SH-SY5Y cell lysate, Neuro-2a cell lysate, Mouse brain tissue lysate, Mouse cerebellum tissue lysate, Rat brain tissue lysate, Rat cerebellum tissue lysate.
Molecular wt :
Predicted band size: 83 kDa
Subcellular location :
Cytosol, nucleus, cytoplasm, neuron projection, presynapse.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:5,000 ;IHC-Fr: 1:200-1:500
more info or order :
HUABIO product webpage
company information
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
headquarters: USA
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!