JMJD6 | HUABIO HA751541 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

JMJD6

catalog :

HA751541

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH15-03

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of JMJD6 on different lysates with Rabbit anti-JMJD6 antibody (HA751541) at 1/5,000 dilution. Lane 1: 293T cell lysate Lane 2: PANC-1 cell lysate Lane 3: HeLa cell lysate Lane 4: SH-SY5Y cell lysate Lane 5: COS-1 cell lysate Lane 6: Neuro-2a cell lysate Lane 7: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 46 kDa Observed band size: 50/110-260 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751541) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of JMJD6 on different lysates with Rabbit anti-JMJD6 antibody (HA751541) at 1/5,000 dilution. Lane 1: HeLa-parental cell lysate Lane 2: HeLa-JMJD6 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 46 kDa Observed band size: 50/110-260 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751541) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunocytochemistry analysis of PC-12 cells labeling JMJD6 with Rabbit anti-JMJD6 antibody (HA751541) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-JMJD6 antibody (HA751541) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

HA751541

Target name :

JMJD6

Species reactivity :

Human,Mouse,Rat,Monkey

Applications :

WB,IF-Cell,IHC-P,FC,IP,ChIP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human JMJD6 aa 1-360.

Uniprot id :

Q6NYC1>SwissProt: Q6NYC1 Human;SwissProt: Q9ERI5 Mouse;SwissProt: Q6AYK2 Rat

Host :

Rabbit

Clone number :

PSH15-03

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

293T cell lysate, PANC-1 cell lysate, HeLa cell lysate, SH-SY5Y cell lysate, COS-1 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, PC-12, human colon cancer tissue, human gastric carcinoma tissue, human lung carcinoma tissue.

Molecular wt :

Predicted band size: 46 kDa

Subcellular location :

Nucleus, nucleoplasm, nucleolus, Cytoplasm.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IF-Cell: 1:100 ;IHC-P: 1:8,000 ;FC: 1:1,000 ;IP: 1-2μg/sample ;ChIP: Use 5 μg for 25 μg of chromatin.

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA751541_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-JMJD6 antibody (HA751541) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751541) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA751541_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue with Rabbit anti-JMJD6 antibody (HA751541) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751541) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA751541_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-JMJD6 antibody (HA751541) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751541) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA751541_7.jpg

Pic legend7 :

Flow cytometric analysis of PC-12 cells labeling JMJD6. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751541, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA751541_8.jpg

Pic legend8 :

JMJD6 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA751541 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751541 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA751541 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA751541 in HeLa cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 3 minutes; ECL: K1801

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA751541_9.jpg

Pic legend9 :

Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells with JMJD6 (HA751541) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

more info or order :

HUABIO product webpage