Histone H3 (tri methyl K36) | HUABIO HA751539 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Histone H3 (tri methyl K36)

catalog :

HA751539

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH15-01

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - paraffin section, dot blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Histone H3 (tri methyl K36) on different lysates with Rabbit anti-Histone H3 (tri methyl K36) antibody (HA751539) at 1/5,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: COS-1 cell lysate (20 µg/Lane) Lane 3: RAW264.7 cell lysate (20 µg/Lane) Lane 4: C6 cell lysate (20 µg/Lane) Lane 5: PC-12 cell lysate (20 µg/Lane) Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751539) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-Histone H3 (tri methyl K36) antibody (HA751539) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751539) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Histone H3 (tri methyl K36) antibody (HA751539) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751539) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA751539

Target name :

Histone H3 (tri methyl K36)

Species reactivity :

Human,Mouse,Rat,Monkey

Applications :

WB,IHC-P,IF-Cell,IP,Dot Blot,ChIP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide corresponding to residues surrounding tri-methyl Lys36 of human histone H3 protein.

Uniprot id :

P68431>SwissProt: P68431 Human;SwissProt: P84243 Human;SwissProt: Q16695 Human;SwissProt: Q6NXT2 Human;SwissProt: Q71DI3 Human;SwissProt: P68433 Mouse;SwissProt: P84228 Mouse;SwissProt: Q6LED0 Rat

Host :

Rabbit

Clone number :

PSH15-01

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, COS-1 cell lysate, RAW264.7 cell lysate, C6 cell lysate, PC-12 cell lysate, human colon cancer tissue, human testis tissue, mouse testis tissue, rat testis tissue, HeLa, RAW264.7, C6.

Molecular wt :

Predicted band size: 15 kDa

Subcellular location :

Nucleus, Chromosome.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IHC-P: 1:2,000 ;IF-Cell: 1:100 ;IP: 1-2μg/sample ;Dot Blot: 1:5,000 ;ChIP: Use 0.5~2 μg for 25 μg of chromatin.

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA751539_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Histone H3 (tri methyl K36) antibody (HA751539) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751539) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA751539_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-Histone H3 (tri methyl K36) antibody (HA751539) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751539) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA751539_6.jpg

Pic legend6 :

Immunocytochemistry analysis of HeLa cells labeling Histone H3 (tri methyl K36) with Rabbit anti-Histone H3 (tri methyl K36) antibody (HA751539) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Histone H3 (tri methyl K36) antibody (HA751539) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA751539_7.jpg

Pic legend7 :

Immunocytochemistry analysis of RAW264.7 cells labeling Histone H3 (tri methyl K36) with Rabbit anti-Histone H3 (tri methyl K36) antibody (HA751539) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Histone H3 (tri methyl K36) antibody (HA751539) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA751539_8.jpg

Pic legend8 :

Immunocytochemistry analysis of C6 cells labeling Histone H3 (tri methyl K36) with Rabbit anti-Histone H3 (tri methyl K36) antibody (HA751539) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Histone H3 (tri methyl K36) antibody (HA751539) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA751539_9.jpg

Pic legend9 :

Histone H3 (tri methyl K36) was immunoprecipitated from 0.2 mg HeLa cell lysate with HA751539 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751539 at 1/20,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA751539 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA751539 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 59 seconds; ECL: K1801

Pic img10 :

https://storage.huabio.cn/huabio/productImg/HA751539_10.jpg

Pic legend10 :

Dot blot analysis of Histone H3 (tri methyl K36) on different peptides with Rabbit anti-Histone H3 (tri methyl K36) antibody (HA751539) at 1/5,000 dilution. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution for 1 hour at room temperature. Lane 1: Unmodified Histone H3 (negative) Lane 2: Mono-Methyl-Histone H3 (Lys36) (negative) Lane 3: Di-Methyl-Histone H3 (Lys36) (negative) Lane 4: Tri-Methyl-Histone H3 (Lys36) (positive) Lane 5: Tri-Methyl-Histone H3 (Lys27) (negative) Proteins loading: 100ng, 25ng, 5ng; Blocking and dilution buffer: 5% NFDM/TBST; Exposure time: 1 minute 59 seconds; ECL: K1802.

Pic img11 :

https://storage.huabio.cn/huabio/productImg/HA751539_11.jpg

Pic legend11 :

Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells with Histone H3 (tri methyl K36) (HA751539) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

more info or order :

HUABIO product webpage