Western blot analysis of IL-27A p28 on different lysates with Rabbit anti-IL-27A p28 antibody (HA751537) at 1/5,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 100ng/mL LPS for 4 hours add 1μg/mL BFA for 3 hours cell lysate Lane 3: RAW264.7 cell lysate Lane 4: RAW264.7 treated with 100nM TPA overnight then treated with 100ng/mL LPS for 4 hours add 1μg/mL BFA for 3 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751537) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.