product summary
Loading...
company name :
HUABIO
product type :
antibody
product name :
Hamartin
catalog :
HA751528
quantity :
100μl
price :
649.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH14-67
reactivity :
human, mouse, rat
application :
western blot, immunoprecipitation
more info or order :
HUABIO product webpage
image
image 1 :
HUABIO HA751528 image 1
Western blot analysis of Hamartin on different lysates with Rabbit anti-Hamartin antibody (HA751528) at 1/20,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: COS-1 cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: MEF cell lysate Lane 6: C2C12 cell lysate Lane 7: PC-12 cell lysate Lane 8: C6 cell lysate Lane 9: Mouse brain tissue lysate Lane 10: Mouse heart tissue lysate Lane 11: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 130 kDa Observed band size: 140 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751528) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
HUABIO HA751528 image 2
Hamartin was immunoprecipitated from 0.2 mg NIH/3T3 cell lysate with HA751528 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751528 at 1/20,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: NIH/3T3 cell lysate (input) Lane 2: HA751528 IP in NIH/3T3 cell lysate Lane 3: Rabbit IgG instead of HA751528 in NIH/3T3 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 50 seconds; ECL: K1801
product information
SKU :
HA751528
Target name :
Hamartin
Species reactivity :
Human,Mouse,Rat,Monkey
Applications :
WB,IP
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within mouse Hamartin aa 651-900.
Uniprot id :
Q92574>SwissProt: Q92574 Human;SwissProt: Q9EP53 Mouse;SwissProt: Q9Z136 Rat
Host :
Rabbit
Clone number :
PSH14-67
Isotype :
IgG
Size :
100μl
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
HeLa cell lysate, 293T cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, MEF cell lysate, C2C12 cell lysate, PC-12 cell lysate, C6 cell lysate, Mouse brain tissue lysate, Mouse heart tissue lysate, Rat brain tissue lysate.
Molecular wt :
Predicted band size: 130 kDa
Subcellular location :
Lysosome membrane, Cytoplasm, cytosol.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:20,000 ;IP: 1-2μg/sample
more info or order :
HUABIO product webpage
company information
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
headquarters: USA
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!