TGF beta Receptor I | HUABIO HA751509 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

TGF beta Receptor I

catalog :

HA751509

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH14-14

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of TGF beta Receptor I on different lysates with Rabbit anti-TGF beta Receptor I antibody (HA751509) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: MCF7 cell lysate Lane 3: SH-SY5Y cell lysate Lane 4: Neuro-2a cell lysate Lane 5: RAW264.7 cell lysate Lane 6: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 56 kDa Observed band size: 50/37 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751509) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

TGF beta Receptor I was immunoprecipitated from 0.2 mg THP-1 cell lysate with HA751509 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751509 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: THP-1 cell lysate (input) Lane 2: HA751509 IP in THP-1 cell lysate Lane 3: Rabbit IgG instead of HA751509 in THP-1 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 7 seconds; ECL: K1801

product information

SKU :

HA751509

Target name :

TGF beta Receptor I

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IP

Conjugate :

Non-conjugated

Uniprot id :

P36897>SwissProt: P36897 Human;SwissProt: Q64729 Mouse;SwissProt: P80204 Rat

Host :

Rabbit

Clone number :

PSH14-14

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

THP-1 cell lysate, MCF7 cell lysate, SH-SY5Y cell lysate, Neuro-2a cell lysate, RAW264.7 cell lysate, C6 cell lysate.

Molecular wt :

Predicted band size: 56 kDa

Subcellular location :

Cell membrane, Cell junction, tight junction, Cell surface, Membrane raft.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IP: 1-2μg/sample

more info or order :

HUABIO product webpage