product summary
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company name :
HUABIO
product type :
antibody
product name :
NDUFA9
catalog :
HA751503
quantity :
100μl
price :
649.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH14-08
reactivity :
human, mouse, rat
application :
western blot, immunoprecipitation
more info or order :
HUABIO product webpage
image
image 1 :
HUABIO HA751503 image 1
Western blot analysis of NDUFA9 on different lysates with Rabbit anti-NDUFA9 antibody (HA751503) at 1/5,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HeLa cell lysate Lane 3: U-2 OS cell lysate Lane 4: Caco-2 cell lysate Lane 5: COS-1 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: Neuro-2a cell lysate Lane 8: C6 cell lysate Lane 9: L6 cell lysate Lane 10: Mouse liver tissue lysate Lane 11: Rat liver tissue lysate Lane 12: Mouse skeletal muscle tissue lysate Lane 13: Rat skeletal muscle tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 43 kDa Observed band size: 36 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751503) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
HUABIO HA751503 image 2
NDUFA9 was immunoprecipitated from 0.2 mg HepG2 cell lysate with HA751503 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751503 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HepG2 cell lysate (input) Lane 2: HA751503 IP in HepG2 cell lysate Lane 3: Rabbit IgG instead of HA751503 in HepG2 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 15 seconds; ECL: K1801
product information
SKU :
HA751503
Target name :
NDUFA9
Species reactivity :
Human,Mouse,Rat,Monkey
Applications :
WB,IP
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human NDUFA9 aa 1-230.
Uniprot id :
Q16795>SwissProt: Q16795 Human;SwissProt: Q9DC69 Mouse;SwissProt: Q5BK63 Rat
Host :
Rabbit
Clone number :
PSH14-08
Isotype :
IgG
Size :
100μl
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
HepG2 cell lysate, HeLa cell lysate, U-2 OS cell lysate, Caco-2 cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, Neuro-2a cell lysate, C6 cell lysate, L6 cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate, Mouse skeletal muscle tissue lysate, Rat skeletal muscle tissue lysate.
Molecular wt :
Predicted band size: 43 kDa
Subcellular location :
Mitochondrion matrix.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:5,000 ;IP: 1-2μg/sample
more info or order :
HUABIO product webpage
company information
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
headquarters: USA
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!