Bid | HUABIO HA751499 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Bid

catalog :

HA751499

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH14-03

reactivity :

human, mouse

application :

western blot, immunoprecipitation

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Bid on different lysates with Rabbit anti-Bid antibody (HA751499) at 1/5,000 dilution. Lane 1: Jurkat cell lysate Lane 2: HT-29 cell lysate Lane 3: A549 cell lysate Lane 4: RAW264.7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751499) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Bid was immunoprecipitated from 0.2 mg RAW264.7 cell lysate with HA751499 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751499 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: RAW264.7 cell lysate (input) Lane 2: HA751499 IP in RAW264.7 cell lysate Lane 3: Rabbit IgG instead of HA751499 in RAW264.7 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 46 seconds; ECL: K1801

product information

SKU :

HA751499

Target name :

Bid

Species reactivity :

Human,Mouse

Applications :

WB,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within mouse Bid aa 51-195.

Uniprot id :

P55957>SwissProt: P55957 Human;SwissProt: P70444 Mouse

Host :

Rabbit

Clone number :

PSH14-03

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Jurkat cell lysate, HT-29 cell lysate, A549 cell lysate, RAW264.7 cell lysate.

Molecular wt :

Predicted band size: 22 kDa

Subcellular location :

Cytoplasm, Mitochondrion membrane, Mitochondrion outer membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IP: 1-2μg/sample

more info or order :

HUABIO product webpage