TRAF1 | HUABIO HA751490 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

TRAF1

catalog :

HA751490

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH13-88

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of TRAF1 on different lysates with Rabbit anti-TRAF1 antibody (HA751490) at 1/10,000 dilution. Lane 1: Raji cell lysate (20 µg/Lane) Lane 2: HEK-293 cell lysate (negative) (20 µg/Lane) Lane 3: Ramos cell lysate (20 µg/Lane) Lane 4: Daudi cell lysate (20 µg/Lane) Lane 5: Rat spleen tissue lysate (40 µg/Lane) Predicted band size: 46 kDa Observed band size: 50 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751490) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

TRAF1 was immunoprecipitated from 0.2 mg Raji cell lysate with HA751490 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751490 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Raji cell lysate (input) Lane 2: HA751490 IP in Raji cell lysate Lane 3: Rabbit IgG instead of HA751490 in Raji cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 10 seconds; ECL: K1801

product information

SKU :

HA751490

Target name :

TRAF1

Species reactivity :

Human,Rat

Applications :

WB,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human TRAF1 aa 1-200.

Uniprot id :

Q13077>SwissProt: Q13077 Human;SwissProt: P39428 Mouse;Entrez Gene: 687813 Rat

Host :

Rabbit

Clone number :

PSH13-88

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Raji cell lysate, Ramos cell lysate, Daudi cell lysate, Rat spleen tissue lysate.

Molecular wt :

Predicted band size: 46 kDa

Subcellular location :

Cytoplasm, cytosol.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:10,000 ;IP: 1-2μg/sample

Advanced Validation :

Relative expression (RE)

more info or order :

HUABIO product webpage