CHCHD2 | HUABIO HA751489 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CHCHD2

catalog :

HA751489

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH13-87

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CHCHD2 on different lysates with Rabbit anti-CHCHD2 antibody (HA751489) at 1/5,000 dilution. Lane 1: A431 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: HepG2 cell lysate (20 µg/Lane) Lane 4: K-562 cell lysate (20 µg/Lane) Lane 5: Jurkat cell lysate (20 µg/Lane) Lane 6: HEK-293 cell lysate (20 µg/Lane) Lane 7: LNCaP cell lysate (20 µg/Lane) Lane 8: Neuro-2a cell lysate (20 µg/Lane) Lane 9: PC-12 cell lysate (20 µg/Lane) Lane 10: Mouse kidney tissue lysate (40 µg/Lane) Predicted band size: 16 kDa Observed band size: 17 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751489) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of A431 cells labeling CHCHD2 with Rabbit anti-CHCHD2 antibody (HA751489) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CHCHD2 antibody (HA751489) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Counterstained with Mitotracker. Nuclear DNA was labelled in blue with DAPI.

image 3 :

Flow cytometric analysis of A431 cells labeling CHCHD2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751489, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA751489

Target name :

CHCHD2

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,FC,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human CHCHD2 aa 1-151.

Uniprot id :

Q9Y6H1>SwissProt: Q9Y6H1 Human;SwissProt: Q9D1L0 Mouse;Entrez Gene: 100910788 Rat

Host :

Rabbit

Clone number :

PSH13-87

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

A431 cell lysate, HeLa cell lysate, HepG2 cell lysate, K-562 cell lysate, Jurkat cell lysate, HEK-293 cell lysate, LNCaP cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, Mouse kidney tissue lysate, A431.

Molecular wt :

Predicted band size: 16 kDa

Subcellular location :

Nucleus, Mitochondrion, Mitochondrion intermembrane space.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IF-Cell: 1:100 ;FC: 1:1,000 ;IP: 1-2μg/sample

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA751489_4.jpg

Pic legend4 :

CHCHD2 was immunoprecipitated from 0.2 mg A431 cell lysate with HA751489 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751489 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: A431 cell lysate (input) Lane 2: HA751489 IP in A431 cell lysate Lane 3: Rabbit IgG instead of HA751489 in A431 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 6 seconds; ECL: K1801

more info or order :

HUABIO product webpage