Phospho-PLC-gamma-1 (S1248) | HUABIO HA751481 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-PLC-gamma-1 (S1248)

catalog :

HA751481

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH13-62

reactivity :

human

application :

western blot, immunoprecipitation

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-PLC-gamma-1 (S1248) on different lysates with Rabbit anti-Phospho-PLC-gamma-1 (S1248) antibody (HA751481) at 1/2,000 dilution. Lane 1: A431 cell lysate Lane 2: A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate Lane 3: A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 149 kDa Observed band size: 149 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751481) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Phospho-PLC-gamma-1 (S1248) was immunoprecipitated from 0.2 mg A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate with HA751481 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751481 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate (input) Lane 2: HA751481 IP in A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate Lane 3: Rabbit IgG instead of HA751481 in A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 7 seconds; ECL: K1801

product information

SKU :

HA751481

Target name :

Phospho-PLC-gamma-1 (S1248)

Species reactivity :

Human

Applications :

WB,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic phosphopeptide corresponding to residues surrounding Ser1248 of human PLC-gamma-1.

Uniprot id :

P19174>SwissProt: P19174 Human

Host :

Rabbit

Clone number :

PSH13-62

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

A431 cell lysate, A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate.

Molecular wt :

Predicted band size: 149 kDa

Subcellular location :

Cell projection, lamellipodium, ruffle.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000-1:10,000 ;IP: 1-2μg/sample

Advanced Validation :

Cell treatment (CT)

more info or order :

HUABIO product webpage