BATF3 | HUABIO HA751471 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

BATF3

catalog :

HA751471

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH13-52

reactivity :

human

application :

western blot, immunoprecipitation, chromatin immunoprecipitation

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of BATF3 on different lysates with Rabbit anti-BATF3 antibody (HA751471) at 1/5,000 dilution. Lane 1: U-937 cell lysate Lane 2: Ramos cell lysate (negative) Lane 3: HDLM2 cell lysate Lane 4: HepG2 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 14 kDa Observed band size: 18 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751471) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

BATF3 was immunoprecipitated from 0.2 mg U-937 cell lysate with HA751471 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751471 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: U-937 cell lysate (input) Lane 2: HA751471 IP in U-937 cell lysate Lane 3: Rabbit IgG instead of HA751471 in U-937 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 3 minutes; ECL: K1801

image 3 :

Chromatin immunoprecipitations were performed with cross-linked chromatin from U-937 cells with BATF3 (HA751471) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

product information

SKU :

HA751471

Target name :

BATF3

Species reactivity :

Human

Applications :

WB,IP,ChIP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human BATF3 aa 1-127.

Uniprot id :

Q9NR55>SwissProt: Q9NR55 Human

Host :

Rabbit

Clone number :

PSH13-52

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

U-937 cell lysate, HDLM2 cell lysate.

Molecular wt :

Predicted band size: 14 kDa

Subcellular location :

Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IP: 1-2μg/sample ;ChIP: Use 0.5~2 μg for 25 μg of chromatin.

Advanced Validation :

Relative expression (RE)

more info or order :

HUABIO product webpage