Bcl10 | HUABIO HA751468 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Bcl10

catalog :

HA751468

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH13-49

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Bcl10 on different lysates with Rabbit anti-Bcl10 antibody (HA751468) at 1/50,000 dilution. Lane 1: A20 cell lysate (20 µg/Lane) Lane 2: RAW264.7 cell lysate (20 µg/Lane) Lane 3: EL4 cell lysate (20 µg/Lane) Lane 4: CTLL-2 cell lysate (20 µg/Lane) Lane 5: Ramos cell lysate (20 µg/Lane) Lane 6: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 26 kDa Observed band size: 31 kDa Exposure time: 14 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751468) at 1/50,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of Raji cells labeling Bcl10 with Rabbit anti-Bcl10 antibody (HA751468) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Bcl10 antibody (HA751468) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunocytochemistry analysis of RAW264.7 cells labeling Bcl10 with Rabbit anti-Bcl10 antibody (HA751468) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Bcl10 antibody (HA751468) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

HA751468

Target name :

Bcl10

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,FC,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human Bcl10 aa 1-233.

Uniprot id :

O95999>SwissProt: O95999 Human;SwissProt: Q9Z0H7 Mouse;SwissProt: Q9QYN5 Rat

Host :

Rabbit

Clone number :

PSH13-49

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

A20 cell lysate, RAW264.7 cell lysate, EL4 cell lysate, CTLL-2 cell lysate, Ramos cell lysate, Rat brain tissue lysate, Raji, RAW264.7.

Molecular wt :

Predicted band size: 26 kDa

Subcellular location :

Cytoplasm, perinuclear region, Membrane raft.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:50,000 ;IF-Cell: 1:100 ;FC: 1:1,000 ;IP: 1-2μg/sample

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA751468_4.jpg

Pic legend4 :

Flow cytometric analysis of Raji cells labeling Bcl10. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751468, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA751468_5.jpg

Pic legend5 :

Flow cytometric analysis of RAW264.7 cells labeling Bcl10. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751468, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA751468_6.jpg

Pic legend6 :

Bcl10 was immunoprecipitated from 0.2 mg RAW264.7 cell lysate with HA751468 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751468 at 1/50,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: RAW264.7 cell lysate (input) Lane 2: HA751468 IP in RAW264.7 cell lysate Lane 3: Rabbit IgG instead of HA751468 in RAW264.7 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 20 seconds; ECL: K1801

more info or order :

HUABIO product webpage