AMH | HUABIO HA751467 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

AMH

catalog :

HA751467

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH13-48

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of AMH on different lysates with Rabbit anti-AMH antibody (HA751467) at 1/5,000 dilution. Lane 1: Mouse ovary tissue lysate Lane 2: Mouse spleen tissue lysate (negative) Lane 3: Mouse testis tissue lysate Lane 4: Rat ovary tissue lysate Lane 5: Rat spleen tissue lysate (negative) Lysates/proteins at 40 µg/Lane. Predicted band size: 59 kDa Observed band size: 59 kDa Exposure time: Lane 1-3: 42 seconds; Lane 4-5: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751467) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Application: IHC-Fr Species: Mouse Site: ovary Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

image 3 :

Application: IHC-Fr Species: Rat Site: ovary Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

product information

SKU :

HA751467

Target name :

AMH

Species reactivity :

Mouse,Rat,Human

Applications :

WB,IHC-Fr,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human AMH aa 411-560.

Uniprot id :

P03971>SwissProt: P03971 Human;SwissProt: P27106 Mouse;SwissProt: P49000 Rat

Host :

Rabbit

Clone number :

PSH13-48

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Mouse ovary tissue lysate, Mouse testis tissue lysate, Rat ovary tissue lysate, mouse ovary tissue, rat ovary tissue.

Molecular wt :

Predicted band size: 59 kDa

Subcellular location :

Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IHC-Fr: 1:500 ;IHC-P: 1:500-1:2,000

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA751467_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse ovary tissue with Rabbit anti-AMH antibody (HA751467) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751467) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA751467_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded rat ovary tissue with Rabbit anti-AMH antibody (HA751467) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751467) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage