ULBP3 | HUABIO HA751458 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

ULBP3

catalog :

HA751458

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH13-03

reactivity :

human

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of ULBP3 on different lysates with Rabbit anti-ULBP3 antibody (HA751458) at 1/5,000 dilution. Lane 1: MDA-MB-231 cell lysate (20 µg/Lane) Lane 2: MCF7 cell lysate (low expression) (20 µg/Lane) Lane 3: SW480 cell lysate (20 µg/Lane) Lane 4: HeLa cell lysate (20 µg/Lane) Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751458) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-ULBP3 antibody (HA751458) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751458) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-ULBP3 antibody (HA751458) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751458) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA751458

Target name :

ULBP3

Species reactivity :

Human

Applications :

WB,IHC-P,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human ULBP3 aa 21-220.

Uniprot id :

Q9BZM4>SwissProt: Q9BZM4 Human

Host :

Rabbit

Clone number :

PSH13-03

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

MDA-MB-231 cell lysate, MCF7 cell lysate, SW480 cell lysate, HeLa cell lysate, human placenta tissue, human stomach tissue.

Molecular wt :

Predicted band size: 28 kDa

Subcellular location :

Cell membrane

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IHC-P: 1:200 ;IP: 1-2μg/sample

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA751458_4.jpg

Pic legend4 :

ULBP3 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA751458 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751458 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA751458 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA751458 in HeLa cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 42 seconds; ECL: K1802

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA751458_5.jpg

Pic legend5 :

Western blot analysis of ULBP3 on different lysates with Rabbit anti-ULBP3 antibody (HA751458) at 1/5,000 dilution. Lane 1: His-tagged Human ULBP3 recombinant protein Lane 2: His-tagged Human ULBP1 recombinant protein Lysates/proteins at 20 ng/Lane. Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751458) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

more info or order :

HUABIO product webpage