product summary
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company name :
HUABIO
product type :
antibody
product name :
CD45
catalog :
HA751457
quantity :
100μl
price :
649.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH13-02
reactivity :
mouse, rat
application :
western blot, immunohistochemistry - frozen section
more info or order :
HUABIO product webpage
image
image 1 :
HUABIO HA751457 image 1
Western blot analysis of CD45 on different lysates with Rabbit anti-CD45 antibody (HA751457) at 1/5,000 dilution. Lane 1: RAW264.7 cell lysate (20 µg/Lane) Lane 2: C2C12 cell lysate (negative) (20 µg/Lane) Lane 3: A20 cell lysate (20 µg/Lane) Lane 2: Neuro-2a cell lysate (negative) (20 µg/Lane) Lane 3: Mouse spleen tissue lysate (40 µg/Lane) Lane 3: Rat spleen tissue lysate (40 µg/Lane) Predicted band size: 145 kDa Observed band size: 250 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751457) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
HUABIO HA751457 image 2
Application: IHC-Fr Species: Mouse Site: spleen Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required
image 3 :
HUABIO HA751457 image 3
Application: IHC-Fr Species: Rat Site: spleen Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required
product information
SKU :
HA751457
Target name :
CD45
Species reactivity :
Mouse,Rat
Applications :
WB,IHC-Fr,IF-Tissue
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within mouse CD45 aa 1-566.
Uniprot id :
P06800>SwissProt: P06800 Mouse;SwissProt: P04157 Rat
Host :
Rabbit
Clone number :
PSH13-02
Isotype :
IgG
Size :
100μl
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
RAW264.7 cell lysate, A20 cell lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate, mouse spleen tissue, rat spleen tissue.
Molecular wt :
Predicted band size: 145 kDa
Subcellular location :
Cell membrane, Membrane raft, Synapse.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:5,000 ;IHC-Fr: 1:500 ;IF-Tissue: 1:200
Advanced Validation :
Relative expression (RE)
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA751457_4.jpg
Pic legend4 :
Application: IF-Tissue Species: Mouse Site: spleen Sample: Paraffin-embedded section Antibody concentration: 1/200
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA751457_5.jpg
Pic legend5 :
Application: IF-Tissue Species: Rat Site: spleen Sample: Paraffin-embedded section Antibody concentration: 1/200
more info or order :
HUABIO product webpage
company information
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
headquarters: USA
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!