product summary
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company name :
HUABIO
product type :
antibody
product name :
PHD2
catalog :
HA751450
quantity :
100μl
price :
649.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH12-74
reactivity :
human
application :
western blot
more info or order :
HUABIO product webpage
image
image 1 :
HUABIO HA751450 image 1
Western blot analysis of PHD2 on different lysates with Rabbit anti-PHD2 antibody (HA751450) at 1/2,000 dilution. Lane 1: 293T cell lysate Lane 2: HepG2 cell lysate Lane 3: SH-SY5Y cell lysate Lane 4: HCT 116 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 46 kDa Observed band size: 50 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751450) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
HUABIO HA751450 image 2
Western blot analysis of PHD2 on different lysates with Rabbit anti-PHD2 antibody (HA751450) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-PHD2 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 46 kDa Observed band size: 50 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751450) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
product information
SKU :
HA751450
Target name :
PHD2
Species reactivity :
Human
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human PHD2 aa 151-426.
Uniprot id :
Q9GZT9>SwissProt: Q9GZT9 Human
Host :
Rabbit
Clone number :
PSH12-74
Isotype :
IgG
Size :
100μl
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
293T cell lysate, HepG2 cell lysate, SH-SY5Y cell lysate, HCT 116 cell lysate.
Molecular wt :
Predicted band size: 46 kDa
Subcellular location :
Cytoplasm, Nucleus.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:2,000
Advanced Validation :
Knockdown (KD)
more info or order :
HUABIO product webpage
company information
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
headquarters: USA
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!