ENPP1 / PC1 | HUABIO HA751440 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

ENPP1 / PC1

catalog :

HA751440

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH12-11

reactivity :

human

application :

western blot, immunohistochemistry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of ENPP1 / PC1 on different lysates with Rabbit anti-ENPP1 / PC1 antibody (HA751440) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: MDA-MB-231 cell lysate Lane 3: Huh7 cell lysate Lane 4: THP-1 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 105 kDa Observed band size: 140 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751440) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-ENPP1 / PC1 antibody (HA751440) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751440) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA751440

Target name :

ENPP1 / PC1

Species reactivity :

Human

Applications :

WB,IHC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human ENPP1 aa 576-925.

Uniprot id :

P22413>SwissProt: P22413 Human

Host :

Rabbit

Clone number :

PSH12-11

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HepG2 cell lysate, MDA-MB-231 cell lysate, Huh7 cell lysate, human liver tissue.

Molecular wt :

Predicted band size: 105 kDa

Subcellular location :

Cell membrane, Basolateral cell membrane, Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

WB:1:5,000 ;IHC:1:1,000

Advanced Validation :

Relative expression (RE)

more info or order :

HUABIO product webpage