product summary
Loading...
company name :
HUABIO
product type :
antibody
product name :
SLAM / CD150
catalog :
HA751437
quantity :
100μl
price :
649.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH12-08
reactivity :
human
application :
western blot, immunoprecipitation
more info or order :
HUABIO product webpage
image
image 1 :
HUABIO HA751437 image 1
Western blot analysis of SLAM / CD150 on different lysates with Rabbit anti-SLAM / CD150 antibody (HA751437) at 1/2,000 dilution. Lane 1: Raji cell lysate (10 µg/Lane) Lane 2: 293T cell lysate (negative) (30 µg/Lane) Lane 3: HUT 102 cell lysate (30 µg/Lane) Lane 4: HeLa cell lysate (negative) (30 µg/Lane) Predicted band size: 37 kDa Observed band size: 75 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751437) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
HUABIO HA751437 image 2
SLAM / CD150 was immunoprecipitated from 0.2 mg Raji cell lysate with HA751437 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751437 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Raji cell lysate (input) Lane 2: HA751437 IP in Raji cell lysate Lane 3: Rabbit IgG instead of HA751437 in Raji cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 30 seconds; ECL: K1801
product information
SKU :
HA751437
Target name :
SLAM / CD150
Species reactivity :
Human
Applications :
WB,IP
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human SLAMF1 aa 1-237.
Uniprot id :
Q13291>SwissProt: Q13291 Human
Host :
Rabbit
Clone number :
PSH12-08
Isotype :
IgG
Size :
100μl
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
Raji cell lysate, HUT 102 cell lysate.
Molecular wt :
Predicted band size: 37 kDa
Subcellular location :
Cell membrane; Secreted.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:2,000 ;IP: 1-2μg/sample
Advanced Validation :
Relative expression (RE)
more info or order :
HUABIO product webpage
company information
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
headquarters: USA
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!