IFT88 | HUABIO HA751417 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

IFT88

catalog :

HA751417

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH11-61

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of IFT88 on different lysates with Rabbit anti-IFT88 antibody (HA751417) at 1/5,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: Jurkat cell lysate Lane 3: HeLa cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: C6 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 93 kDa Observed band size: 93 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751417) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of IFT88 on different lysates with Rabbit anti-IFT88 antibody (HA751417) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: A549 cell lysate Lane 3: HepG2 cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: C6 cell lysate Lane 6: COS-1 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 93 kDa Observed band size: 93 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751417) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-IFT88 antibody (HA751417) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751417) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA751417

Target name :

IFT88

Species reactivity :

Human,Mouse,Rat,Monkey

Applications :

WB,IHC-P,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human IFT88 aa 575-824.

Uniprot id :

Q13099>SwissProt: Q13099 Human;SwissProt: Q61371 Mouse;Entrez Gene: 305918 Rat

Host :

Rabbit

Clone number :

PSH11-61

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HEK-293 cell lysate, Jurkat cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, C6 cell lysate, A549 cell lysate, HepG2 cell lysate, COS-1 cell lysate, human testis tissue, rat testis tissue, NIH/3T3, C6.

Molecular wt :

Predicted band size: 93 kDa

Subcellular location :

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole, Cell projection, cilium, cilium basal body, flagellum.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000-1:5,000 ;IHC-P: 1:1,000 ;IF-Cell: 1:50

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA751417_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-IFT88 antibody (HA751417) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751417) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA751417_5.jpg

Pic legend5 :

Immunocytochemistry analysis of NIH/3T3 cells labeling IFT88 with Rabbit anti-IFT88 antibody (HA751417) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IFT88 antibody (HA751417) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA751417_6.jpg

Pic legend6 :

Immunocytochemistry analysis of C6 cells labeling IFT88 with Rabbit anti-IFT88 antibody (HA751417) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IFT88 antibody (HA751417) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

more info or order :

HUABIO product webpage