FAM38A / PIEZO1 | HUABIO HA751411 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

FAM38A / PIEZO1

catalog :

HA751411

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH11-49

reactivity :

mouse

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of FAM38A / PIEZO1 on different lysates with Rabbit anti-FAM38A / PIEZO1 antibody (HA751411) at 1/2,000 dilution. Lane 1: 293T transfected with mPiezo1 cell lysate Lane 2: 293T transfected with mPiezo2 cell lysate (negative) Lysates/proteins at 10 µg/Lane. Exposure time: 2 minutes 50 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751411) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of 293T cells labeling FAM38A / PIEZO1 with Rabbit anti-FAM38A / PIEZO1 antibody (HA751411) at 1/200 dilution. 293T cells, transfected with GFP-tagged mouse Piezo1 (top, positive) / tag-free mouse Piezo2 (bottom, negative) expression vector, respectively, were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FAM38A / PIEZO1 antibody (HA751411) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

product information

SKU :

HA751411

Target name :

FAM38A / PIEZO1

Species reactivity :

Mouse

Applications :

WB,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within mouse PIEZO1 aa 2,214-2,457.

Uniprot id :

E2JF22>SwissProt: E2JF22 Mouse

Host :

Rabbit

Clone number :

PSH11-49

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

293T transfected with mPiezo1 cell lysate.

Molecular wt :

Predicted band size: 292 kDa

Subcellular location :

Endoplasmic reticulum membrane, Endoplasmic reticulum-Golgi intermediate compartment membrane, Cell membrane, Cell projection, lamellipodium membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IF-Cell: 1:200

Advanced Validation :

Cell treatment (CT)

more info or order :

HUABIO product webpage