HIP2 / LIG | HUABIO HA751403 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

HIP2 / LIG

catalog :

HA751403

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH11-24

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of HIP2 / LIG on different lysates with Rabbit anti-HIP2 / LIG antibody (HA751403) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: 293T cell lysate (20 µg/Lane) Lane 3: HCT 116 cell lysate (20 µg/Lane) Lane 4: Daudi cell lysate (20 µg/Lane) Lane 5: Jurkat cell lysate (20 µg/Lane) Lane 6: K-562 cell lysate (20 µg/Lane) Lane 7: U-2 OS cell lysate (20 µg/Lane) Lane 8: COS-1 cell lysate (20 µg/Lane) Lane 9: C2C12 cell lysate (20 µg/Lane) Lane 10: NIH/3T3 cell lysate (20 µg/Lane) Lane 11: PC-12 cell lysate (20 µg/Lane) Lane 12: Mouse brain tissue lysate (40 µg/Lane) Lane 13: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 22 kDa Observed band size: 25 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751403) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of HeLa cells labeling HIP2 / LIG with Rabbit anti-HIP2 / LIG antibody (HA751403) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HIP2 / LIG antibody (HA751403) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunocytochemistry analysis of NIH/3T3 cells labeling HIP2 / LIG with Rabbit anti-HIP2 / LIG antibody (HA751403) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HIP2 / LIG antibody (HA751403) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

HA751403

Target name :

HIP2 / LIG

Species reactivity :

Human,Mouse,Rat,Monkey

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human HIP2 aa 1-200.

Uniprot id :

P61086>SwissProt: P61086 Human;SwissProt: P61087 Mouse;Entrez Gene: 289623 Rat

Host :

Rabbit

Clone number :

PSH11-24

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, 293T cell lysate, HCT 116 cell lysate, Daudi cell lysate, Jurkat cell lysate, K-562 cell lysate, U-2 OS cell lysate, COS-1 cell lysate, C2C12 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, HeLa, NIH/3T3, PC-12, human brain tissue, human liver tissue, mouse heart tissue, mouse liver tissue, rat liver tissue.

Molecular wt :

Predicted band size: 22 kDa

Subcellular location :

Cytoplasm

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IF-Cell: 1:100 ;IHC-P: 1:1,000 ;FC: 1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA751403_4.jpg

Pic legend4 :

Immunocytochemistry analysis of PC-12 cells labeling HIP2 / LIG with Rabbit anti-HIP2 / LIG antibody (HA751403) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HIP2 / LIG antibody (HA751403) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA751403_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-HIP2 / LIG antibody (HA751403) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751403) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA751403_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-HIP2 / LIG antibody (HA751403) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751403) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA751403_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-HIP2 / LIG antibody (HA751403) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751403) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA751403_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-HIP2 / LIG antibody (HA751403) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751403) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA751403_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-HIP2 / LIG antibody (HA751403) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751403) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/HA751403_10.jpg

Pic legend10 :

Flow cytometric analysis of HeLa cells labeling HIP2 / LIG. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751403, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img11 :

https://storage.huabio.cn/huabio/productImg/HA751403_11.jpg

Pic legend11 :

Flow cytometric analysis of NIH/3T3 cells labeling HIP2 / LIG. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751403, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img12 :

https://storage.huabio.cn/huabio/productImg/HA751403_12.jpg

Pic legend12 :

Flow cytometric analysis of PC-12 cells labeling HIP2 / LIG. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751403, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage